从海南哥纳香中分离的一种新化合物──海南哥纳香醇甲的抗肿瘤作用  

STUDIES ON THE ANTICANCER EFFECT OF HOWIINOL A,A NEW COMPOUND ISOLATED FROM GONIOTHALAMUS HOWII

作　　者：何剑华[1] 叶玉梅[1] 徐承熊[1] 

机构地区：[1]中国医学科学院中国协和医科大学药物研究所

出　　处：《药学学报》1998年第7期493-497,共5页Acta Pharmaceutica Sinica

摘　　要：体外用细胞生长曲线测定法、ＭＴＴ试验、软琼脂集落形成分析法，及体内对移植性肿瘤实验，研究了海南哥纳香醇甲（ＧＨＭ１０）的抗肿瘤作用，结果表明：ＧＨＭ１０对肿瘤细胞有较强的抑制作用，ＩＣ５０在２μｇ·ｍｌ－１左右；对正常细胞影响较小，骨髓祖细胞的敏感性则更低；耐药的ＫＢ／ＶＣＲ２００细胞及其亲本ＫＢ细胞具有相似的敏感性。ＧＨＭ１０对ＨＬ６０细胞无分化诱导作用。ＧＨＭ１０对实体型肝癌Ｈ２２小鼠。The effect of howiinol A (GHM10) on the growth of several cancer and normal cells were studied, using the methods of cell growth curve determination, MTT test and softagar colony formation assay. The results showed that GHM10 exhibited potent inhibitory effect on cancer cells with an IC50 of 2 μg·ml-1 approximately. Normal cells, especially bone marrow progenitor cells, were shown to be less sensitive to GHM10. A drugresistant cell line, KB/VCR200, was found to be as sensitive as its parental KB cells. GHM10 did not induce HL60 cell differentiation. In murine transplantable tumors, GHM10 was found to have significant therapeutic effect on mice bearing solid tumor hepatoma H22, Lewis lung carcinoma and S180 ascites.

关 键 词：海南哥纳香醇甲 海南哥纳香 抗肿瘤作用 

分 类 号：R931.71[医药卫生—生药学] R979.1[医药卫生—药学]