胆红素对大鼠海马CA_3区长时程增强影响的研究  

The effects of bilirubin on long term potentiation of rat hippocampal CA_3 region

作　　者：张炼[1] 刘皖君[1] 

机构地区：[1]同济医科大学附属同济医院儿科

出　　处：《中华儿科杂志》1998年第8期461-464,共4页Chinese Journal of Pediatrics

摘　　要：目的研究胆红素的神经系统毒性。方法用电生理方法观察了急性注射胆红素对大鼠海马ＣＡ３区长时程增强的影响情况。结果对照组给予高频刺激后第１、３、５、１０、１５、３０、４５、６０、９０和１２０分钟群体峰电位变化率显著增加（ＤｕｎｎｅｔＴ临界值为２．８０９，Ｐ＜０．０５），而两个实验组的群体峰电位变化率在刺激前后均无明显改变（Ｐ＞０．０５）；高频刺激后第３、５、１０、４５、６０和１２０分钟，对照组群体峰电位变化率较当时的两个实验组明显增大（Ｐ＜０．０５），在其他时刻则未发现明显改变，未发现两个实验组之间的群体峰电位变化率存在差别。Objective To determine the neurotoxicity of bilirubin. Methods Electrophysiological method was used to observe the effects of acute bilirubin injection on long term potentiation (LTP) of rat hippocampal CA_3 region. Results The results showed that, contrary to the two experimental groups (bilirubin 30 mg/kg and 60 mg/kg), the control group had significantly increased change rate of population spike at the time of 1, 3, 5, 10, 15, 30, 45, 60, 90 and 120 minutes after highfrequency stimulation (P<0.05, critical value of Dunnett′s T=2.809). At the time of 3, 5, 10, 45, 60 and 120 minutes after highfrequency stimulation, the control group′s change rate of population spike was significantly increased as compared to that of the two experimental groups at the same time points (P<0.05), at other moments during observation no significant differences were found between the three groups.Conclusion This study has confirmed that bilirubin could depress the induction of LTP and affect the ability of learning and memory of children.

关 键 词：胆红素 海马 电生理学 高胆红素血症 新生儿 

分 类 号：R722.170.2[医药卫生—儿科]