中华慈姑中具有抗菌活性的对映-玫瑰烷和对映-贝壳杉烷二萜(英文)  

Antibacterial ent-Rosane and ent-Kaurane Diterpenoids from Sagittaria trifolia var.sinensis

作　　者：刘雪婷[1,2] 施瑶[1] 梁敬钰[1] 闵知大[1] 

机构地区：[1]中国药科大学天然药物化学教研室,南京210009 [2]Department of Chemistry,Department of Microbiology,University of Wisconsin-La Crosse

出　　处：《中国天然药物》2009年第5期341-345,共5页

摘　　要：目的:研究中华慈姑中的抗菌活性成分。方法:通过各种硅胶柱层析和凝胶柱层析进行分离和纯化。分离得到的化合物通过一维和二维的核磁技术以及化学方法进行结构解析。结果:从中华慈姑中分离得到10个化合物,并测试了化合物1-3抗3种口腔细菌的活性。结论:化合物1是一个新的对映-玫瑰烷二萜苷,化合物3显示了很强的抗两种口腔细菌(Streptococus mutans和Actinomyces naeslundiis)的活性,MIC均为15.6μg·mL-1。化合物1的活性较弱,对上述两种口腔细菌的MIC均为62.5μg·mL-1。AIM： To investigate the antibacterial components from Sagittaria trifolia var. sinensis. METHODS： The separation was performed over silica gel and Sephadex LH-20 column. The structures of the obtained compounds were determined on the basis of NMR data and chemical methods. RESULTS： Ten diterpenoids were isolated. Sagittine H （1）, sclareol （2）, and 19-β-L-3＇-acetoxyarabinofuranosyl- ent-kaur-16-ene-19-oate （3） were evaluated for their abilities to inhibit the growth of three oral pathogens, Streptococcus mutans ATCC 25175, Actinomyces naeslundiis ATCC 12104, and Actinobacillus actinomycetemcomitans ATCC 43717, using minimum inhibitory concentration （MIC） assays. CONCLUSIONS： sagittine H （1） is a new ent-rosane glycoside and demonstrated MIC of 62.5μg-mL^-1 against both S. mutans and A. naeslundiis. Compound 3 exhibits strong activity against S. mutans and A. naeslundiis with MIC of 15.6 μg.mL^-1 for both.

关 键 词：中华慈姑 对映-玫瑰烷 二萜 抗菌活性 

分 类 号：R284[医药卫生—中药学]