污叉丝孔菌菌丝体培养物中烷基苷类化合物(英文)  

Alkyl Glycosides from Mycelial Cultures of Dichomitus squalens

作　　者：谢海辉[1] 徐新亚[1] 丹阳[1] 魏孝义[1] 

机构地区：[1]中国科学院植物资源保护与可持续利用重点实验室中国科学院华南植物园,广州510650

出　　处：《中国天然药物》2009年第5期390-393,共4页

基　　金：supported by the National Natural Science Foundation of China (No.20672114);the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry (No.20071108)~~

摘　　要：目的:研究污叉丝孔菌Dichomitus squalens(Karst) Reid菌丝培养物中的化学成分。方法:在广东省鼎湖山采集污叉丝孔菌子实体,通过组织培养分离获得菌丝体,然后用多个含有YMG培养基和小麦的10.5L三角瓶进行培养。菌丝体培养物用乙醇提取和溶剂分部,正丁醇溶解部位经柱层析和高效液相色谱分离纯化,用光谱分析法鉴定化合物结构。结果:分离获得了9个化合物,分别鉴定为乙基β-D-吡喃木糖苷(1),乙基β-D-吡喃木糖(1→4)-β-D-吡喃木糖苷(2),正丁基β-D-吡喃木糖苷(3),正丁基β-D-吡喃木糖(1→4)-β-D-吡喃木糖苷(4),乙基β-D-吡喃葡萄糖苷(5),乙基α-L-呋喃阿拉伯糖苷(6),正丁基α-L-呋喃阿拉伯糖苷(7),2-去氧-D-核糖-1,4-内酯(8),2-去氧-D-核酸甲酯(9)。结论:以上化合物系首次从污叉丝孔菌菌丝体培养物中分离获得。AIM： To study the chemical constituents of mycelial cultures of Dichomitus squalens （Karst） Reid, a common white rot Basidiomycetous fungus. METHOD： Mycelia were isolated from tissue plugs of a fruiting body collected in Dinghu Mountain, Guangdong, and fermented on a large scale in 10.5-L flasks containing YMG medium and wheat grains. The EtOH extract of the mycelial cultures was fractionated, and the n-BuOH-soluble fraction was sepa- rated by column chromatography and HPLC. Chemical structures were elucidated on the basis of spectroscopic analysis. RESULT： Nine compounds were isolated and elucidated as ethyl β-D-xylopyranoside （1）, ethyl β-D-xylopyranosyl-（1→4）-β-D-xylopyranoside （2）, n-butyl β-D-xylopyranoside （3）, n-butylβ-D-xylopyranosyl-（1→4）-β-D-xylo- pyranoside （4）, ethyl β-D-glucopyranoside （5）, ethyl a-L-arabinofuranoside （6）, n-butyl a-L-arabinofuranoside （7）, 2-dexoy-D-ribono-1,4-1actone （8）, and 2-dexoy-D-ribonic acid methyl ester （9）. CONCLUSION： These compounds have been obtained from mycelial cultures of D. squalens for the first time.

关 键 词：污叉丝孔菌 菌丝体培养物 乙基苷 丁基苷 

分 类 号：Q936[生物学—微生物学]