离子液体双水相中茜素红S与牛血清白蛋白作用研究  

Study on the Interaction Between Alizarin Red S and Bovine Serum Albumin in Ionic Liquid Aqueous Two-phase System

作　　者：朱陈银[1] 邓凡政[1] 高贺军[1] 

机构地区：[1]淮北煤炭师范学院化学与材料科学学院,安徽淮北235000

出　　处：《淮北煤炭师范学院学报（自然科学版）》2009年第3期29-31,共3页Journal of Huaibei Coal Industry Teachers College(Natural Science edition)

基　　金：安徽省教育厅自然科学研究重点项目(KJ2008A123)

摘　　要：在离子液体四氟硼酸1-丁基-3-甲基咪唑([Bmim]BF4)和NaH2PO4形成的双水相体系中,研究茜素红S(ARS)与牛血清白蛋白(BSA)生成复合物光谱行为和溶液酸度,盐浓度,离子液体用量,共存物质等对体系复合物测定影响.离子液体用量1.5mL,磷酸二氢钠2.0g,ARS1.0mL,蛋白质溶液1.0mL,pH6时ARS与BSA复合物吸光度较大.离子液相中复合物最大吸收波长位于531nm,比单纯ARS红移109nm;摩尔吸光系数(ε)为2.32×104L·mol-1·cm-1.用加入不同类型表面活性剂方法,初步探讨了ARS与BSA之间的作用机理.使用后的离子液体经过简单处理可以重复使用.In ionic liquid [Bmim] BF4（1 -butyl- 3- methylimidazoliumtetrafluoroborate） and NaH2PO4 aqueous two -phase system （ATPS）, the spectroscopic behaviour of the compound of alizarin red S （ARS） and bovine serum albumin （BSA） was investigated. Effects of different acidity, quantities of ionic liquid and salt and coexistent matter on the determination of system were discussed. In the ionic liquid aqueous two -phase system of a pH 6, 1.0 mL ARS, 1.5 mL [Bmim] BF4 and 2.0 g NaH2PO4, the compound formed by ARS and BSA had high absorbance with the maximum absorption of 531 nm. The results showed that compared to ARS spectra, the maximum wavelength of compound shifted to the red by 109 nm, and the apparent molar absorptivity （ε）of 2. 32×10^4L·mol^-1cm^-1. With different surfactant, the interaction mechanism of ARS and BSA were discussed initiatively. The ionic liquid could be repeatedly used after simple treatment.

关 键 词：离子液体 茜素红S 牛血清白蛋白 

分 类 号：O657.32[理学—分析化学]