嗜麦芽窄食单胞菌磺胺耐药基因和Ⅰ类整合酶基因的研究  

Sulfadiazine-resistante gene and Int I gene in stenotrophomonas maltophilia

作　　者：邓笑伟[1] 姚秀萍[2] 刘长庭[3] 

机构地区：[1]武警总医院特需健康医学中心,北京100039 [2]武警总医院首保临床部,北京100039 [3]解放军总医院南楼呼吸科,北京100853

出　　处：《武警医学》2009年第9期803-805,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的了解临床分离嗜麦芽窄食单胞菌磺胺耐药基因(sulⅠ)和Ⅰ类整合酶基因(intⅠ)的存在情况。方法收集临床分离的32株嗜麦芽窄食单胞菌,采用微量肉汤稀释法测定12种抗菌药物的MIC;PCR法扩增sulⅠ基因和Ⅰ类整合酶基因。结果32株嗜麦芽窄食单胞菌7株表现对复方磺胺甲噁唑(SXT)耐药(21.9%),10株菌Ⅰ类整合酶基因阳性(31.3%),18株菌sulⅠ阳性(56.3%)。结论嗜麦芽窄食单胞菌对复方磺胺甲噁唑耐药可能与Ⅰ类整合子存在有关,Ⅰ类整合子作为耐药性传播的重要机制可能将使磺胺类药物的应用受到一定限制。Objective To investigate the distribution of the sulfadiazine - resistant gene （sul Ⅰ ） and Int Ⅰ gene in Stenotro- phomonas maltophilia. Methods Minimal inhibitory concentration was determined by microdosis broth dilution method. Sul Ⅰ gene and Int ] gene were detected by PCR methods in 32 clinical strains of Stenotrophomonas rrtaltophilia. Results Seven isolates of 32 Stenotrophomonas maltophilia showed resistance to trimethoprim - sulfamethoxazole （21.9%）, and Int I gene was positive in 10 strains, sul Ⅰ was positive in 18 strains. Conclusions Class I integron is likely associated with high drug resistance to trimethoprim - sulfamethoxazole in Stenotrophomonas maltophilia. High spread of class Ⅰ integrons will potentially limit the use of trimethoprim - sulfamethoxazole for therapy of severe Stenotrophomonas maltophilia infection.

关 键 词：磺胺耐药基因 整合酶 嗜麦芽窄食单胞菌 

分 类 号：R978.2[医药卫生—药品]