静脉麻醉药预处理对鼠脑缺血损伤皮质区TNF-α的影响  

作　　者：李秀华[1] 程晓燕[2] 张岩[3] 

机构地区：[1]潍坊医学院麻醉学系危重病医学教研室,261042 [2]潍坊市人民医院麻醉科 [3]潍坊医学院附属医院麻醉科

出　　处：《当代医学》2009年第28期6-8,共3页Contemporary Medicine

摘　　要：目的观察异丙酚与依托咪酯脂肪乳剂预处理对大鼠短暂性脑缺血损伤时皮层额顶叶区肿瘤坏死因子(TNF-α)表达的影响及神经细胞形态学的改变。方法利用结扎双侧颈总动脉合并血压下降法模型造成大鼠短暂性脑缺血损伤,实验共分四组:假手术组(SH组)、缺血模型组(ISC组)、异丙酚预处理组(PPC组)及依托咪酯预处理组(EPC组),免疫组化分析大脑皮质额顶叶区神经细胞内TNF-α阳性表达情况。结果大鼠脑缺血30min后,与SH组相比,ISC组TNF-α的阳性表达明显增加(P<0.01),EPC组和PPC组较ISC组TNF-α阳性表达均减少(P<0.01),PPC组与EPC组比较,前者作用大于后者(P<0.01)。结论在脑缺血缺氧损伤的早期即可发生大脑皮层额顶叶区细胞因子TNF-α的阳性表达增加;异丙酚和依托咪酯预处理均可减少细胞因子TNF-α的阳性表达,且异丙酚的作用优于依托咪酯。Objective To observe the change of TNF-α in the fronto-apical lobe of cerebral cortex in rats with transient cerebral ischemia and anoxia injure. Methods The model of transient cerebral ischemia injury was made by the ligation of bilateral carotid arteries combining the descending of the blood pressure. Forty-eight male Wistar rats were stochastically divided into the sham operated group（SH）, the ischemia group（ISC）, propofol preconditioning group（PPC） and etomidate preconditioning group（EPC） （n=12 for each group）. The quantity and total area of TNF-α expression in cerebral cortex investigated by immune histochemisty staining and assaying. Results Immune Histochemistry staining and assaying showed that the positive expression of TNF-α in the EPC and PPC groups were markedly decreased with that in ISC group （P〈0.01）. Quantitative image analysis indicated that the quantity of its positive expression in PPC group was different from EPC group （P〈0.01）.Conclusion There was the increasing of cytokine TNF- αexpression in the earlier period of cerebral ischemia. Intravenous anesthetics： propofol and etomidate pretreatments could decrease the toxicity of cytokine TNF- α. The effect of propofol was better than etomidate＇s.

关 键 词：异丙酚 依托咪酯 短暂性脑缺血 TNF—α 

分 类 号：R96[医药卫生—药理学]