一种高效细胞电融合仪的研制  

Development of high-efficiency cell-electrofusion device

作　　者：杨静[1,2] 杨军[1] 许蓉[1] 曹毅[1,3] 胡宁[1] 郑小林[1] 

机构地区：[1]重庆大学生物工程学院,重庆400030 [2]重庆通信学院军事信息工程系,重庆400035 [3]重庆城市管理职业学院电子信息工程系,重庆400055

出　　处：《传感器与微系统》2009年第9期86-88,共3页Transducer and Microsystem Technologies

基　　金：国家高技术研究发展计划资助项目(2006AA04Z343);国家自然科学基金资助项目(30870661;30500120;30770569)

摘　　要：针对现有细胞电融合仪的不足,研究开发了一种基于微系统技术的高效自动细胞电融合仪。该仪器将MEMS工艺加工的电融合芯片与电融合信号发生器集成为一体,通过精确的结构设计和信号产生来控制细胞的位置。由于芯片的结构特点,使融合控制电极间距减小,从而降低了对融合电压的要求。融合装置可选用多种不同尺寸规格的电极,而且,细胞融合进程可以直接进行显微观察和操作。融合实验表明:该装置可以提高细胞融合效率,融合率达到40%。Aimed at the deficiency in the existing cell-elctrofusion devices, a microsystem technology-based highefficiency and automatic cell-electrofusion device is developed. MEMS technique fabricated cell-electrofusion chip is integrated with signal generator, in which precisely designed microstructure and signal are used for locating cells. In this chip, small distance between one pair of microelectrodes reduces required voltage. Many differentsize microelectrodes can be chosen in this device. Cell fusion can also be observed and manipulated under microscope. According to the result in the on-chip cell-fusion experiments, up to 40 % fusion efficiency is obtained.

关 键 词：电融合 微流控 生物芯片 微电极 

分 类 号：Q813.2[生物学—生物工程]