青风藤醇提液及其有效成分防治吗啡戒断症状的实验研究  

Study on the Detoxification of Alcohol Extracts from Orientvine and Its Effective Component on Withdrawal Syndromes of Morphine

作　　者：张国梅[1] 莫志贤[2] 王彩云[2] 

机构地区：[1]广州军区联勤部药品仪器检验所,广东广州510500 [2]南方医科大学,广东广州510515

出　　处：《中药材》2009年第9期1414-1418,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:观察青风藤醇提液及其有效成分青藤碱对吗啡成瘾后催促戒断症状、单胺类神经递质及吗啡依赖神经细胞内Ca2+的影响,研究青风藤和青藤碱的吗啡依赖戒断症状作用并探讨其机制。方法:通过吗啡依赖豚鼠离体回肠实验及吗啡依赖小鼠催促戒断实验,观察青风藤醇提液及青藤碱预先作用对吗啡依赖豚鼠离体回肠及吗啡依赖小鼠戒断症状的影响。以剂量递增法形成吗啡依赖大鼠模型,采用Rf5000双波长荧光测定法观察青风藤醇提液及青藤碱对吗啡依赖大鼠下丘脑单胺类神经递质去甲肾上腺素(NA)、多巴胺(DA)、5-羟色胺(5-HT)的影响。建立吗啡依赖神经细胞模型,Fluo-3着染细胞后,应用流式细胞仪测定青风藤醇提液及青藤碱对神经细胞内Ca2+浓度的影响。结果:青风藤醇提液及青藤碱均能不同程度地抑制吗啡依赖豚鼠回肠的戒断性收缩反应,作用呈剂量依赖性,能显著抑制吗啡依赖小鼠的戒断反应,降低吗啡依赖大鼠戒断后骤增的单胺类神经递质。青风藤醇提液及青藤碱能使神经细胞内Ca2+浓度显著升高,抑制纳洛酮催促戒断引起的吗啡依赖神经细胞内Ca2+降低。结论:青风藤醇提液及青藤碱对吗啡依赖戒断症状具有一定治疗作用,其作用机理可能是抑制单胺类神经递质释放和调节细胞内Ca2+浓度。Objective： To observe the effect of alcohol extracts from orientivne and its effective component sinomenine on withdrawal syndromes and neurotransmitter of morphine-abstinent mice and on intracellular calcium level in morphine-dependent neuronalcells line. To study the detoxificiation of alcohol extracts from orientvine and sinomenine on morphine-dependent animal and explore the mechanism of its effect. Methods ： The effect of alcohol extracts from orientivne and sinomenineon on abstinent syndromes was observed by experiment study on morphine-dependent ex vivo ileum from guinea pigs and morphine-dpendent mice. The morphine-dependent model mice were established by injection on dosage increasing by degrees. The hypothalamic monomine neurotrasmitters such as NA, DA, 5-HT were tested by fluorospectrophotometry. Morphine-dependent cell line was established by administering morphine at different doses into the culture medium. The cells were stained with fluo-3 and the intracellular calcium level was measured by flow cytometry. Results ： Alcohol extracts from orientvine and sinomenine could alleviate withdrawal contractile response of morphine-dependent ex vivo ileum from guinea pigs and withdrawal syndromes of morphine-dependent mice, decrease the concentration of the neurotransmitters, and elevate the intracellular calcium level and inhibit the decreasing of Ca^2＋ induced by naloxone. Conclusions ： Alcohol extracts from orientvine and sinomenine have some effects on withdrawal syndromes which may be related to inhibiting neurotransmitters and the regulation of intracellular calcium concentration.

关 键 词：青风藤 青藤碱 吗啡依赖 戒断症状 单胺类神经递质 细胞内钙 

分 类 号：R285.5[医药卫生—中药学]