四君子汤及其拆方对D-半乳糖诱导衰老小鼠的免疫调节作用  

The Immunomodulatory Effects of Sijunzi Decoction and Its Disassembled Prescription on D-galactose-Induced Aging Mice

作　　者：成文利[1] 王修银[2] 江振友[3] 潘竞锵[2] 董军[3] 邝少松[4] 饶子亮[4] 

机构地区：[1]中山大学附属第六医院,广东广州510655 [2]广州市中医医院,广东广州510130 [3]暨南大学医学院,广东广州510632 [4]广东省医学实验动物中心,广东广州510180

出　　处：《中药材》2009年第9期1425-1429,共5页Journal of Chinese Medicinal Materials

基　　金：暨南大学国家中医药管理局病理生理重点实验室开放课题基金(51207029);广东省中医药局建设中医药强省科研课题(1060117);广州市中;西医结合课题(2005B030)

摘　　要：目的:观察四君子汤及其拆方对D-半乳糖(D-gal)诱导小鼠衰老模型的免疫调节作用。方法:用D-半乳糖诱导小鼠衰老模型,采用花环和脾淋巴细胞转化试验(MTT法)测定模型小鼠红细胞免疫和T细胞免疫功能;采用试剂盒对丙二醛(MDA)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)等氧化抗氧化指标进行检测。结果:四君子汤能显著提高D-半乳糖诱导衰老小鼠的T淋巴细胞转化能力(P<0.05),显著提高C3b花环率(P<0.05),显著降低IC花环率(P<0.05),并能显著提高其血清SOD及GSH-Px活性(P<0.01),显著降低血清MDA含量(P<0.05)。结论:四君子汤党参、白术对D-半乳糖(D-gal)诱导衰老模型小鼠有免疫调节作用,而茯苓、甘草无明显作用。Objective ： To observe the immunomodulatory effects of codonopsis, atractylodes macrocephala, tuceahoe, broiled licorice and sijunzi decoction on D-galaetose-induced aging mice. Methods： The models of aging mice were induced by D-galactose, the garlands and splenic lymhocyte transformation test （MTT） were used to determine the ability of erythroeytes immune and lymphocytes conversion; The content of maleic dialdehyde （MDA） , activity of superoxide dismutase（SOD） and glutathione pemfidase（GSH-Px） in serum were dectected. Results： Sijunzi decoction and its disassembled prescription codonopsis could significantly increase the ability of T lymphocyte transformation（ P 〈 0. 05 ） ; Atractylodes macrocephala and sijunzi decoction could significantly enhance C3b garlands ratio （P 〈 0. 05 ） and decrease IC garlands ratio（ P 〈 0. 05 ） on D-galactose-induced aging mice. Sijunzi decoction significantly increased the activity of SOD and GSH-Px （P 〈 0.01 ）, and decreased the content of MDA in serum（ P 〈 0.05 ）. Conclusion： Sijunzi decoction and its disassembled prescription codonopsis, atraetylodes maerocephala can improve the immunomodulatory effects on D-galactose-indueed aging mice; but tuceaboe and boriled icoriee have no obvious effects.

关 键 词：四君子汤 D-半乳糖 免疫调节 

分 类 号：R285.5[医药卫生—中药学]