短瓣金莲花总黄酮大孔树脂纯化工艺研究  

Study on Purification of Total Flavones from Trollius ledebouri by Macroporous Resin

作　　者：周欣[1] 范国荣[2] 

机构地区：[1]南京军区福州总医院药学科,福建福州350025 [2]第二军医大学药学院药物分析教研室,上海200433

出　　处：《中药材》2009年第9期1450-1454,共5页Journal of Chinese Medicinal Materials

摘　　要：目的:研究短瓣金莲花总黄酮的大孔树脂纯化工艺条件。方法:通过对短瓣金莲花总黄酮在3种大孔吸附树脂上的吸附与解吸附特性的比较,筛选最佳树脂,并对该树脂的吸附特性及各影响因素进行系统研究,采用正交试验及单因素考察等方法对纯化工艺条件进行优选。结果:以D101大孔吸附树脂为分离载体,样品液浓度为0.5 g/mL,pH6.0,上样量为药材∶树脂=1∶1(W/W),上样后静态吸附1 h,先用4 BV水洗脱除杂,再用6 BV 30%乙醇解吸,所得产品中总黄酮平均含量为60%以上。Objective： To study the optimal technologies for the purification of total flavones from Trollius. ledebouri with macroporous resin. Methods ： Static and dynamic adsorption-desorption methods were adopted to choose the optimal type of resin. The adsorption function of D101 macroporous resin and the effects of sample concentration, pH, flow rate and eluant etc. were studied. Then the orthogonal design L9 （34） was used to select the optimum purification process conditions. Results： The appropriate technological conditions were as follows： the sample concentration was 0. 5 g/mL, pH6.0, the ratio of sample to D101 macroporous resin was 1：1 （ W/W）, 4 BV of water was used as purificant and 6 BV of 30% alcohol was used as eluant. Under these conditions, the average content of total flavonoids extract was over 60%.

关 键 词：短瓣金莲花 总黄酮 大孔树脂 

分 类 号：R284.2[医药卫生—中药学]