Effects of 2,4-D and 6-BA on Callus Induction and Plantlet Regeneration from Mature Embryos of Hsien Rice  

2,4-D和6-BA对籼稻成熟胚愈伤组织诱导和植株再生的影响

作　　者：苗春波[1] 万志刚[1] 孙丙耀[1] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123

出　　处：《Agricultural Science & Technology》2009年第4期22-26,共5页农业科学与技术（英文版）

基　　金：Supported by the National Natural Science Foundation (30571049)~~

摘　　要：[ Objective] In order to study the effects of 2,4-D and 6-BA on callus cultivation from mature embryos of hsien rice. [ Method] 2,4-D and 6-BA were set at different concentrations in callus induction and differentiation mediums to study their effects on callus induction, seedling formation and regenerated seedlings rooting. [ Result] In the callus induction medium treated with 0.5 mg/L 2,4-D, the callus induction effects on the varieties like Jiayu 948, Yanghui 559, Yangxian 6547, Zhong＇erruanzhan, Minghui 86, Guanghui 998 and Zunxian 3 were the best; If 0.2 mg/L 6-BA was added into the callus induction medium containing the optimum level of 2,4-D, there was no obvious effect on induction rate of callus, but the differentiation and seedling of callus were inhibited; If the concentration of 6-BA was reduced appropriately in the differentiation medium, the seedling rate of callus would be not only no decreased but increased, meanwhile the quality of regenerated plants would be improved. [ Conclusion] The study results provided some references for the reasonable uses of 2,4-D and 6-BA in callus culture of hsien rice.1材料与方法1．1材料供试材料为12个籼稻品种的成熟种子（籽实）。其中，籼小粘、嘉育948、中二软占、湘晚籼1号、遵籼3号、中鉴1006个品种由国家水稻种质资源中期库提供，扬稻6号、扬籼6547、盐恢559、镇恢084、广恢998、明恢86六个品种由江苏太湖农业科学研究所提供。

关 键 词：Hsien rice CALLUS 2 4-D 6-BA 

分 类 号：S511.21[农业科学—作物学] S567.239