Identification of the Parentage of Corn Variety Using SSR Markers  

SSR标记鉴定玉米品种亲子关系的研究

作　　者：沈童伟[1,2] 陆徐忠[2] 刘勋辉[1,2] 李莉[2] 杨剑波[2] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]安徽农业科学院水稻所,安徽合肥230031

出　　处：《Agricultural Science & Technology》2009年第4期55-60,共6页农业科学与技术（英文版）

基　　金：Supported by the National Standard Plan(20051079-T-469)~~

摘　　要：[Objective] The study was to explore the molecular interpretation standards on parentage in the seeds of corn variety. [Method] With 16 hybrids and their parents and 202 inbred elites as materials for screening primers, the artificial groups of two standard diad and two standard triad were respectively established as the verification materials. Genomic DNA of seedlings was extracted by using CTAB method. 137 pairs of SSR primers were selected for SSR amplification and product detection, which was used for the parentage identification of maize varieties. [ Result] Twenty pairs of corn primers with high polymorphism information content （ PIC value）, clearly amplified bands and good reproducibility were screened from 137 pairs of corn SSR primers tested. The identification results of using SSR molecular were consistent with the actual situation. [ Conclusion] It is feasible to identify the parentage of maize variety using SSR markers.1材料与方法1．1材料选取我国主要推广的16个玉米杂交组合及其双亲以及主要杂种优势群中的202个骨干自交系，其中185份自交系为中国玉米核心种质材料。种子严格隔离保纯，纯度99．9％以上。种子30℃发苗取其部分叶片提取DNA。

关 键 词：MAIZE SSR marker Corn primer Parentage identification 

分 类 号：S513[农业科学—作物学] S962.1