Study on Establishment of cpSSR Marker Technology and Optimization of Its Reaction System in Jatropha curcas Linn  

麻疯树cpSSR标记技术的建立与体系优化

作　　者：向倩[1] 周兰英[1] 万静[1] 张旭[1] 雷宝盛[1] 金银春[1] 冯毅[1] 于绪任[1] 赵晓英[1] 

机构地区：[1]四川农业大学林学院,四川雅安625014

出　　处：《Agricultural Science & Technology》2009年第4期61-64,74,共5页农业科学与技术（英文版）

基　　金：Supported by National Scientific and Technical Supporting Project ofStudies on Superior Species Selecting and Breeding Technique ofJatropha curcasLinn(2007BAD50B01)~~

摘　　要：[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.1材料与方法1．1材料采自我国西南10个地区的麻疯树野生居群的枝条扦插（表1），摘取幼叶用于提取总DNA。

关 键 词：Jatropha curcas Linn cpSSR-PCR Optimization of reaction system 

分 类 号：S792.99[农业科学—林木遗传育种]