Distribution of H-FABP mRNA in Hybrids of Songliao Black Pig×Sus scrofa  

松辽黑猪×野猪杂交猪H-FABP mRNA基因的组织分布研究

作　　者：秦莹[1] 张永宏[1] 刘同欣[1] 马倩[1] 朱银莉[1] 孙博兴[1] 赵志辉[1] 张嘉保[2] 高妍[1,2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林大学实验动物中心,吉林长春130062

出　　处：《Agricultural Science & Technology》2009年第4期65-67,71,共4页农业科学与技术（英文版）

基　　金：Supported by the National High Technology Research and Develop-ment Program of China(No.2008AA10Z136);the Program of Liaoning Provincial Science-Technology Department(20070567)~~

摘　　要：[ Objective] This study was to investigate the distribution of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa, so as to provide references for revealing the physiological functions of this gene. [Method] With the mRNA from different tissues as template, RT-PCR amplification was carried out for cloning cDNA of hybrid pig, which was then used for PCR reaction using specific primers; the amplification products were separated by gel electrophoresis and analyzed by sequencing for detecting the distribution pattern of H-FABP mRNA in hybrids of Songliao black pig x Sus scrofa. [ Result] H-FABP expressed in all the twelve tissues including subcutaneous fat, abdominal fat, mammary gland, Iongissimus dorsi muscle, dorsal deltoid muscle, heart, liver, spleen, lung, kidney, ileum and duodenum. [ Conclusion] The wide distribution of H-FABP gene suggests that its functions are important and multiple.1材料与方法1．1材料松辽黑猪X野猪杂交猪（含野猪血缘50％）组织样品采自吉林省农业科学院畜牧分院，采集的皮下脂肪、腹脂、乳腺、背最长肌、臂三头肌、心脏、肝脏、脾脏、肺脏、肾脏、回肠、十二指肠共计12种组织样品迅速置于液氮中，实验室-80℃保存。

关 键 词：Hybrids of Songliao black pig x Sus scrofa H-FABP mRNA Tissue distribution 

分 类 号：S828[农业科学—畜牧学]