Genetic Polymorphism in GDF8 Region of HU Sheep Based on Microsatellite DNA  

利用微卫星标记分析湖羊GDF8区域的遗传多样性

作　　者：吴文忠[1] 钱建共[1] 陈玲[1] 孙伟[2] 

机构地区：[1]江苏省苏州市畜牧兽医站,江苏苏州215128 [2]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《Agricultural Science & Technology》2009年第4期68-71,共4页农业科学与技术（英文版）

基　　金：Supported by the State Scientific Basic Research Platform Program( No. 2005DKA21101);China Postdoctoral Science Foundation(No. 20080430470);Natural Science Foundation of Jiangsu Prov-ince of China (BK2007556);the National high-tech R&D program(863 program)(No.2006AA10Z198);Key Projects in the NationalScience &Technology Pillar Program during the Eleventh Five-Year Plan Period(No.2006BAD13B08 );Support Foundation of Chinaduring the 11th Five-Year Plan Period (No. 2008BADB2B04);BasicNatural Science Foundation for Colleges and Universities in JiangsuProvince (NK051039);Jiangsu Government Scholarship for Over-seas Studies Project;Qing Lan Project of Colleges and UniversitiesJiangsu Province;the New Century Talent Project of Yangzhou University in China~~

摘　　要：[ Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. [ Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level （PIC 〉0.5）. [ Conclusion ] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.1材料与方法1．1试验材料与DNA提取2007年，在江苏省苏州市种羊场随机选取成年羊88只，采取耳组织提取DNA，-20℃保存。并记录湖羊的初生重、断奶重和6月龄重。

关 键 词：Hu sheep GDFS Microsatellite DNA Growth performance 

分 类 号：S826[农业科学—畜牧学]