Analysis of Protein Expression Patterns of Silkworm Jinqiu and Its Cross Parents  

作　　者：YU Fang YANG Hui-juan LI Jian-ying DING Nong ZHOU Zhong-hua YE Jian ZHANG Jin-wei DUAN Jia-long ZHONG Bo-xiong 

机构地区：[1]College of Animal Sciences, Zhejiang University, Hangzhou 310029, P.R. China [2]Huzhou Academy of Agriculture Sciences, Huzhou 313000, P.R.China [3]College of Life Sciences, Anhui University, Hefei 230036, P.R.China

出　　处：《Agricultural Sciences in China》2009年第9期1130-1137,共8页中国农业科学（英文版）

基　　金：supported by the National Basic Re-search Program of China (973 Program,2005CB121003);the National High-Tech Research and Development Program of China (863 Program,2006AA10A118);the Doctoral Fund of Ministry of Education of China (20070335148)

摘　　要：The differences of protein expression between the improved cross breeding race Jinqiu and its parents were analyzed to discuss the gene construction, and to form a base for illuminating the molecular mechanisms of successful cross breeding in silkworm. Protein samples from silk gland, hemolymph, and midgut were separated by 2-dimensional gel electrophoresis （2-DE）. In the three tissues the matched protein spots between Jinqiu and its cross parents were approximately 70% with approximately 30% specific protein spots. In the matched protein spots, 9-24% was differentially expressed representing up- and down-regulated expression. These specific protein spots might be either the newly appeared, which were produced from the genic interaction of cross parents＇ genes in cross breeding, or posttranscriptionally modified, which were produced from the different modifications on the same original proteins. These results indicate that it is important for a new successful breed, by cross breeding, relying on the actions of some newly produced functional proteins from genic interaction, in addition to marshaling excellent genes of cross parents.The differences of protein expression between the improved cross breeding race Jinqiu and its parents were analyzed to discuss the gene construction, and to form a base for illuminating the molecular mechanisms of successful cross breeding in silkworm. Protein samples from silk gland, hemolymph, and midgut were separated by 2-dimensional gel electrophoresis （2-DE）. In the three tissues the matched protein spots between Jinqiu and its cross parents were approximately 70% with approximately 30% specific protein spots. In the matched protein spots, 9-24% was differentially expressed representing up- and down-regulated expression. These specific protein spots might be either the newly appeared, which were produced from the genic interaction of cross parents＇ genes in cross breeding, or posttranscriptionally modified, which were produced from the different modifications on the same original proteins. These results indicate that it is important for a new successful breed, by cross breeding, relying on the actions of some newly produced functional proteins from genic interaction, in addition to marshaling excellent genes of cross parents.

关 键 词：SILKWORM PROTEOME cross breeding silk gland HEMOLYMPH MIDGUT 

分 类 号：S88[农业科学—特种经济动物饲养]