甘蓝小孢子植株倍性简易鉴定方法研究  

Studies on Facility Identification Method for Ploidy of Cabbage Microspore Plant

作　　者：邓耀华[1] 熊艺[2] 周国林[1] 汪爱华[1] 

机构地区：[1]武汉市蔬菜科学研究所,湖北武汉430065 [2]武汉工程大学,湖北武汉430073

出　　处：《中国蔬菜》2009年第18期46-50,共5页China Vegetables

摘　　要：以编号为12、22的甘蓝材料(F1)经小孢子培养得到的植株为试材,先利用流式细胞仪对供试材料进行倍性鉴定,再利用普通光学显微镜观察已通过倍性鉴定的双单倍体与单倍体的气孔保卫细胞,以测定其大小与叶绿体数。结果表明:气孔保卫细胞叶绿体数与甘蓝倍性高低呈正比关系,甘蓝双单倍体气孔保卫细胞叶绿体数变异幅度为12～17,单倍体气孔保卫细胞叶绿体数的变异幅度为6～10;同一材料双单倍体与单倍体气孔保卫细胞叶绿体数的比值≈2,两者数值差异非常显著直观,易于辨别。因此观测气孔保卫细胞叶绿体数是鉴定甘蓝小孢子植株倍性的简易有效手段。The cuhivars obtained by microspore culture in cabbage of two F） cultivars No. 12 and No. 22 were studied. Firstly, ploidys of the two F1 cultivars were detected by using flow cytometry. Secondly, the size of stomata protective cells was observed and the chloraplast number of doubled haploid euhivars and haploid cuhivars with ploidy were counted and identified. The results show that there is a positive relationship between the chloraplast number of stomata protective cells and ploidy of cabbage. The chloraplast numbers of stomata protective cells in doubled haploid cuhivars vary from 12 to 17 and that in haploid euhivars vary from 6 to 10. Thus the ratio of the chloraplast number of stomata protective cells in doubled haploid eultivars to that in haploid cultivars is about 2, and there is big difference between the chloraplast number of stomata protective cells in doubled haploid cuhivars and of haploid cuhivars. So observing the chloraplast number of stomata protective cells could be an easy and effective mean to identify ploidy of cabbage.

关 键 词：甘蓝 小孢子植株 倍性鉴定 气孔保卫细胞 叶绿体数 

分 类 号：S635[农业科学—蔬菜学]