蜘蛛丝的结构性能及表达策略研究进展  

Progress of Studies on the Structure and Properties of Spider Silk and its Expression Strategies

作　　者：郑青亮[1] 蒋彩英[1] 张耀洲[1] 

机构地区：[1]浙江理工大学生命科学学院生物化学研究所,杭州310018

出　　处：《蚕业科学》2009年第3期685-691,共7页ACTA SERICOLOGICA SINICA

基　　金：浙江省重中之重开放基金(编号SWYX0806);国家高技术研究发展计划"863"项目(编号2007AA021703;2007AA100504);国家重点基础研究发展计划"973"项目(编号2005CB121006);国家自然科学基金项目(编号30740015)

摘　　要：蜘蛛丝是一种天然蛋白质纤维,具有高强度、高弹性、高断裂能等机械性能以及显著的可降解性、组织相容性等生物学特性,在生物医学、材料、纺织和军事装备等领域均有重大潜在应用价值。利用原核或真核表达系统表达蜘蛛丝蛋白可以大量获取蜘蛛丝。综述了蜘蛛丝蛋白序列结构特征以及蜘蛛丝蛋白在大肠杆菌、酵母、植物、动物细胞、家蚕等表达系统中的表达策略研究进展,并重点阐述应用家蚕表达系统表达蜘蛛丝蛋白的策略,可供规模化生产蜘蛛丝蛋白参考。Spider silk is a natural protein fiber with mechanical properties such as high strength, high flexibility, high fracture energy, and with biological characteristics of easy degradation and histocompatibility which have significant potential application value in the biomedical, material, textile and military areas. Using prokaryotic or eukaryotic expression system to express spider silk protein can yield Targe quantity of spider silk. This paper reviewed the structural characteristics of spider silk protein and the progress of spider silk expression strategies in E. coil, yeast, plants, animal cells and silkworm expression system, and focused on the new ideas of spider silk expression strategy in the silkworm expression system. This can provide a reference for future large-scale production of spider silk proteins.

关 键 词：蜘蛛丝 机械性能 表达策略 家蚕表达系统 

分 类 号：Q789[生物学—分子生物学]