广西鼠疫指示动物犬血清IHA、ELISA检测鼠疫F1抗体的一致性研究  

Agreement of IHA and ELISA in the detection of F1 antibody to Yersinia pestis in indicator animals of plague in Guangxi

作　　者：黄德蕙[1] 梁江明[1] 曾竣[1] 秦石英[1] 廖驰真[1] 李寿生[1] 陈达宗[1] 韦锦平[1] 鲁翠芳[1] 周树武[1] 罗寿军 吴声荣 李红军[3] 覃刚[3] 农雪飞 热娜[4] 海荣[5] 

机构地区：[1]广西壮族自治区疾病预防控制中心,广西南宁530028 [2]广西隆林县疾病预防控制中心,广西隆林533400 [3]广西西林县疾病预防控制中心,广西西林533500 [4]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830002 [5]中国疾病预防控制中心传染病预防控制所,北京102206

出　　处：《国外医学（医学地理分册）》2009年第3期117-119,共3页Foreign Medical Sciences：Section of Medgeography

基　　金：国家"十五"科技攻关项目(No.2004BA718B07-01)

摘　　要：目的了解应用IHA、ELISA检测鼠疫F1抗体的一致性,评价方法的优劣,以便更好地制订鼠疫监测的方法及防治策略。方法对广西鼠疫监测点隆林、西林县的犬血清使用IHA及ELISA两种试验方法,并进行一致性研究,评价IHA与ELISA方法的可靠性。结果IHA检测鼠疫F1抗体阳性率为0,ELISA检测鼠疫F1抗体的总阳性率为4.4%(12/273);IHA与ELISA之间的一致性为95.6%,其Kappa值等于0,呈轻度一致。结论间接血凝试验方法特异、操作简便是传统的鼠疫监测手段之一;酶联免疫吸附试验具有敏感性高、特异性强、操作简便等特点,且与间接血凝试验有轻度一致性,适宜在鼠疫监测中推广应用。Objective To evaluate the performance of IHA and ELISA for the detection of F1 antibody to Yersinia pestis, in order to develop appropriate surveillance and prevention strategies. Methods IHA and ELISA tests were used in the dog serum samples in Longlin County and Xilin County, and then the agreement between the two tests was measured to assess their reliability. Results None of the serum samples were positive to F1 antibody in the IHA test, while 12/273 （4.4%） were positive in the ELISA test. The agreement rate between the two assays was 95.6%, and the Kappa value was zero, showing a low agreement. Conclusions IHA remains one of the clas- sical tests in plague surveillance for its specificity and simplicity. ELISA is highly sensitive, specific and easy to perform. Furthermore, it has a low agreement with IHA and therefore it＇s useful for plague surveillance.

关 键 词：鼠疫 指示动物 一致性研究 

分 类 号：R254.8[医药卫生—中医内科学]