银杏叶愈伤组织培养及黄酮积累的正交实验优化  

Optimization of Growth and Flavonoids Formation of Callus in Ginkgo Biloba L. Leaves by Orthogonal Experiment

作　　者：胡燕梅[1] 唐兴国[1] 宋鹏飞[1] 梅双喜[1] 张治华[1] 

机构地区：[1]武汉生物工程学院生物工程系,湖北武汉430415

出　　处：《化学与生物工程》2009年第9期59-62,共4页Chemistry & Bioengineering

基　　金：湖北省教育厅科学技术研究资助项目(B20094008);武汉市高校科学研究资助项目(2008K079)

摘　　要：采用正交实验研究了基本培养基、NAA浓度、6-BA浓度、植酸(PA)浓度对银杏叶愈伤组织继代培养及黄酮积累的影响,并测定了愈伤组织生长曲线。结果表明,银杏叶愈伤组织继代培养的最佳组合为:基本培养基N6,NAA1.0mg·L-1,6-BA1.0mg·L-1,0.05%PA。各因素对愈伤组织生长影响的主次顺序为:NAA浓度>基本培养基>PA浓度>6-BA浓度;愈伤组织黄酮积累量最佳组合为:基本培养基B5,NAA1.0mg·L-1,6-BA1.0mg·L-1,0.3%PA,各因素对愈伤组织黄酮积累量影响的主次顺序为:PA浓度>基本培养基>NAA浓度>6-BA浓度。愈伤组织生长曲线基本呈"S"型。Th＇rough orthogonal experiment, the effects of elemental medium,NAA concentration, 6-BA concentration,phytacid（15A） concentration on growth and flavonoids formation of callus in Ginkgo biloba L. leaves were investigated and the growth curve of callus was determined. The results showed that, for growth of callus, the optimum culture conditions were as follows：N6 as elemental medium,NAA concentration 1.0 mg·L^-1 ,6-BA concentration 1.0 mg ·L^-1, PA concentration 0. 05 %, and the effect order was NAA concentration〉elemental medium^PA concentration〉-BA concentration;for flavonoids formation of callus, the optimum culture conditions were as follows：B5 as elemental medium,NAA concentration 1.0 mg ·L^-1 ,6-BA concentration 1.0 mg ·L^-1,PA concentration 0.3% ,and the effect order was PA concentration〉elemental medium〉NAA concentration〉-BA concentration. The growth curve of callus exhibited the type of “S”.

关 键 词：银杏(Ginkgo biloba L.) 正交实验 愈伤组织 黄酮 

分 类 号：R284.1[医药卫生—中药学]