尖塘鳢属鱼类线粒体16S rRNA基因序列变异及分子系统进化  

Sequences Variation and Molecular Phylogeny of Mitochondrial 16S rRNA Gene of the Genus Oxyleotris

作　　者：王茜[1] 齐兴柱[1] 骆剑[1] 范小勇[1] 王小刚[1] 尹绍武[1] 陈国华[1] 吴光明 

机构地区：[1]海南大学海洋学院海南省热带水生生物技术重点实验室热带生物资源教育部重点实验室,海南海口570228 [2]海南断山渔业有限公司,海南三亚572013

出　　处：《海南大学学报（自然科学版）》2009年第3期245-251,共7页Natural Science Journal of Hainan University

基　　金：海南省重点科技计划项目(070119);三亚市重点科技项目(080115);海南大学重点学科建设项目资助

摘　　要：为探讨尖塘鳢属鱼类的系统发育关系，笔者通过PCR法得到云斑尖塘鳢、线纹尖塘鳢和尖头塘鳢的线粒体16SrRNA基因部分序列（569bp），并结合从GenBank中下载的平头沙塘鳢、溪鳢及刺盖塘鳢的16SrRNA基因部分同源序列，分析了6种塘鳢科鱼类的系统发育关系．运用MAGA4．0软件分析可知，塘鳢鱼类6个种之间存在142个变异位点，其中简约信息位点57个，转换／颠换之比为1．6，表明塘鳢科鱼类16SrRNA基因序列转换／颠换未达饱和；6种塘鳢科鱼类之间的序列差异在0．054～0．147之间，其中平头沙塘鳢和刺盖塘鳢的序列差异最大（0．147），线纹尖塘鳢与云斑尖塘鳢的序列差异最小（0．054），且种间序列差异不大．通过NJ，ME，MP系统树的建立，表明线纹尖塘鳢与云斑尖塘鳢的亲缘关系最近，且尖塘鳢属与塘鳢属鱼类的同源性不高，验证了传统分类学把尖塘鳢独立成属的科学性．To investigate the molecular phylogeny relationship of the genus Oxyleotris, partial sequences mitochondria 16S rRNA gene of three species fish （ Oxyeleotris marmorata, Oxyeleotris lineolata and Eleotris oxycephala） were cloned by PCR methods. Together with homologous 16S rRNA gene sequences of Odontobutis platycephala, Rhyacichthys aspro and Eleotris acanthopoma from GenBank, the mitoehondria 16S rRNA sequences of six species fish were analyzed by MEGA 4.0 software. The results showed that 142 sites were variable among all the partial mitochondria 16S rRNA sequences of the six species fish, in which there were 57 phylogenetically inform active sites. The ratio of transition and transversion was 1.6, which meant that the base substitution does not reach soturation. The results also indicated that the differentiation of the six sequences ranged from O. 054 to 0. 147. The differentiation of sequences between Odontobutis platycephala and Eleotris acanthopoma was the biggest （0. 147）, and the differentiation of sequences between Oxyeleotris lineolata and Oxyeleotris mar- morata was the smaller （0.054）. But the sequence differentiation among the six species fish was not significant. Based on phylogenetic trees （N J, ME and MP） analysis, the results showed that Oxyeleotris marmorata and Oxyeleotris lineolata were closely related, and the homology between Oxyleotris and Eleotridae was lower, which supported the separation of Oxyleotris from Eleotridae in traditional taxonomic treatment.

关 键 词：云斑尖塘鳢 线纹尖塘鳢 尖头塘鳢 线粒体DNA 16S rRNA 

分 类 号：Q959.483[生物学—动物学]