半夏提取物对气道黏液高分泌的影响  

Airway Mucus Hypersecretion in Rats Is Inhibited by the Extracts of Pinellia

作　　者：邓青南[1] 周建龙[1] 郭振辉[1] 张萍[1] 

机构地区：[1]广州军区广州总医院,广东广州510010

出　　处：《中国呼吸与危重监护杂志》2009年第5期477-481,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：广东省科技计划项目(编号:2008B030301197)

摘　　要：目的探讨半夏提取物(EP)对脂多糖诱导的大鼠气道黏液高分泌模型的干预作用。方法30只Wistar大鼠随机分为5组(n=6),分别为空白组、模型组、低剂量EP组、中剂量EP组及高剂量EP组。模型组和EP组经气道注入脂多糖(LPS)复制大鼠气道黏液高分泌模型。低、中、高剂量EP组连续4 d分别给予管饲EP 10、30及60 g/kg;采用免疫组化法检测肺内气道的黏蛋白5AC(MUC5AC)蛋白表达,RT-PCR法检测肺组织MUC5AC mRNA、水通道蛋白5(AQP-5)mRNA;ELISA法检测BALF中的TNF-α浓度。结果大鼠气道上皮MUC5AC蛋白和肺组织MUC5AC mRNA的表达在模型组中均明显高于空白组(P<0.05);在低剂量及中剂量EP组较模型组稍降低(P>0.05),仍显著高于空白组(P<0.05);在高剂量EP组显著低于模型组(P<0.05)。大鼠AQP-5 mRNA表达在模型组显著低于空白组(P<0.05);在低剂量及中剂量EP组较模型组稍升高(P>0.05),仍较空白组显著降低(P<0.05);在高剂量EP组显著高于模型组(P<0.01)。BALF中TNF-α浓度与肺组织MUC5AC mRNA的表达呈正相关(r=0.948,P<0.05),肺组织AQP-5 mRNA与MUC5AC mRNA及TNF-α的表达呈负相关(r=-0.955,P<0.05;r=-0.909,P<0.05)。结论高剂量的EP能明显抑制大鼠气道上黏液高分泌状态。Objective To investigate the effects of extracts of Pinellia （EP） on a rat model of airway mucus hypersecretion induced by LPS. Methods Thirty Wisatr rats were randomly divided into 5 groups, ie. a blank group, a model group, and three EP groups treated with different doses of EP. There were 6 rats in each group. Airway mucus hypersecretion model was established by intratracheally instillation of LPS in the model group and three EP groups. The rats in three EP groups were orally administered with EP at dosages of 10 g/kg, 30 g/kg and 60 g/kg respectively for 4 days. The expression of Mucin 5AC （MUC5AC） protein in airway was assayed by immunohistochemistry. The mRNA expressions of MUC5AC and Aquaporin-5 （AQP-5） in lung tissue were detected by RT-PCR. ELISA technique was performed to detect TNF-α in bronchoalveolar lavage fluid（BALF）. Results LPS significantly stimulated the mRNA and protein expression of MUC5AC in lung and TNF-α level in BALF, and inhibited the expression of AQP-5 mRNA in lung. The EP at dosages of 10 g/kg and 30 g/kg had little effect on mucus hypersecretion. While 60 g/kg of EP could significantly inhibited the expression of MUC5AC, and decreased the release of TNF-α in BALF. The AQP-5 mRNA was also up-regulated by 60 g/kg of EP. The expression of MUC5AC mRNA was positively correlated with level of TNF-α（ r = 0. 948 ,P 〈 0. 05 ） ; AQP-5 mRNA was negatively correlated withMUC5AC mRNA and TNF-α（r= -0.955,P〈0.05; r= -0.909,P〈0.05）. Conclusion EP （60 g/kg） can significantly attenuated airway mucus hypersecretion in rats.

关 键 词：黏液高分泌 半夏 黏蛋白5AC 水通道蛋白5 

分 类 号：R285.5[医药卫生—中药学]