椒目油A2通过抑制EOS浸润和骨髓粒系动员减轻哮喘小鼠气管炎症反应  

Influence of Jiaomu oil A2 on eosinophil manifold and CD34^+ with marrow granule system mobilization in bronchia asthma mice

作　　者：王俊琴[1] 王胜春[1] 田卫斌[1] 曹增发[1] 

机构地区：[1]第四军医大学西京医院药剂科,陕西西安710032

出　　处：《中国病理生理杂志》2009年第9期1708-1714,共7页Chinese Journal of Pathophysiology

基　　金：陕西省中医管理局科学基金资助项目(No.2005-076)

摘　　要：目的:观察椒目油A2对哮喘支气管嗜酸性粒细胞(EOS)浸润与CD34+细胞及骨髓粒系动员的影响。方法:腹腔注射20%氢氧化铝[Al(OH)3]+10%卵蛋白(OVA)混合液致敏与雾化吸入1%OVA诱发BALB/c小鼠哮喘模型。在激发10 d并同时给药治疗后,小鼠被处死,瑞氏染色检测肺灌洗液(BALF)炎性细胞及骨髓造血细胞系变化,原位杂交(ISH)检测肺组织和骨髓白细胞介素-5(IL-5)mRNA和嗜酸性粒细胞趋化因子(EON)mRNA表达,免疫组化检测肺组织和骨髓中IL-5、EON的蛋白表达,HE和荧光染色观察肺组织炎症细胞浸润和CD34+细胞。结果:椒目油A2和泼尼松显著地减轻肺组织支气管炎性反应的病变程度如组织肿胀、重构、增生、上皮细胞脱落等,抑制变应源致敏刺激机体所致EOS增多和浸润,明显地减少哮喘小鼠气管周围及骨髓中EOS密度,抑制骨髓中幼粒细胞向嗜酸性粒细胞分化,同时支气管区及骨髓中CD34+IL-5、CD34+IL-5R、CD34+CCR-3细胞数显著减少,这与肺组织内IL-5、IL-4、GM-CSF等细胞因子水平降低有关。结论:椒目油A2减轻实验性哮喘小鼠气道炎症反应,其机制可能与其抑制支气管EOS增多和骨髓粒系动员相关。AIM： To observe the influence of Jiaomu oil A2 on eosinophil manifold and CD34^＋ with marrow granule system mobilization in bronchial asthma mice. METHODS： The asthmatic mouse model was established by sensitization and challenge of the animals with 20% Al（OH）3 ＋ 10% ovalbumen （OVA）. After the mice were excitated for 10 d and giving medical therapy at the same time, the mice were executed, the bronchial - alveolar lavage inflammatory ceils and the hemocytopoiesis cells were examined using Wrish - Giemsa staining. The expressions of interleukin - 5 （ IL - 5 ） and eotaxin （EON） in lung tissue and marrow were examined by in situ hybridization. The expression of IL -5 and EON albumen in lung tissue and marrow were detected by immunohistochemistry. The inflammatory cell infiltration and CD34^＋ cells in lung tissue were also observed by HE and immunofluorescence staining. RESULTS： Both Jiaomu oil A2 and prednisone significantly attenuated the pathological process degree of bronchial inflammatory reaction such as tissue swelling, reconstruction, hyperplasia, and epithelial cell shedding, and inhibited eosinophil count and infiltration caused by stimulation of allergic effect. Moreover, the two drugs markedly lessen the eosinophil density in tracheal surrounding tissue and marrow in asthma mice and depressed the differentiation of marrow myelocyte to eosinophil. Finally, the apparent decrement of CD34 ＋ IL-5, CD34 ＋ IL-5R, CD34 ＋ CCR-3 cells in bronchial tissue and marrow showed some relationship with the downregulation of IL - 5, IL - 4, GM - CSF in lung tissue. CONCLUSION ： The effect of Jiaomu oil on airway inflammation in asthma mice is associated with inhibiting the mobilization of eosinophil and marrow granule system.

关 键 词：椒目油 气道炎症 哮喘 骨髓 

分 类 号：R284.1[医药卫生—中药学]