环孢素A对白色念珠菌诱导产生IL-17的抑制作用  

Cyclosporin A inhibits the production of IL-17 induced by Candida albicans

作　　者：权志博[1] 刘昀[2] 李琴[2] 乔丹[2] 吴长有[2] 

机构地区：[1]陕西中医学院医学技术系,咸阳712046 [2]中山大学中山医学院免疫学教研室,广州510080

出　　处：《中国免疫学杂志》2009年第9期771-773,779,共4页Chinese Journal of Immunology

基　　金：国家重点基础研究发展规划(973)资助项目(2007CB512404)

摘　　要：目的:研究正常人外周血单个核细胞(PBMCs)在白色念珠菌刺激条件下,环孢素A(CsA)对IL-17产生的影响。方法:PBMCs用白色念珠菌+抗CD28单克隆抗体(mAb)刺激后,用ELISA法检测培养上清中IL-17及IFN-γ的水平。同时利用ELISPOT法检测IL-17产生细胞的频率。结果:CsA对白色念珠菌刺激条件下诱导的PBMCs产生的IL-17与IFN-γ均为剂量依赖性的抑制,且对IL-17的抑制率高于IFN-γ,而且CsA在培养的早期和晚期均能抑制IL-17与IFN-γ的产生。同时,与单独白色念珠菌刺激相比,加入CsA后,能抑制IL-17产生细胞的频率。结论:CsA剂量依赖性抑制白色念珠菌诱导产生IL-17,进一步证明了CsA用于治疗白色念珠菌感染的疗效。Objective：To study the effect of Cyelosporin A （CsA） on the production of IL-17 by human PBMCs induced by Candida albieans. Methods： PBMCs were cultured with Candida albieans plus anti-CD28 mAb in the presence or absence of CsA at different concentrations. After stimulation for 3 days, the supematants were harvested and assayed for production of IL-17 and IFN-γ by ELISA. The frequency of IL-17-produeing cells was examined at single cell level by ELISPOT. Results：After stimulation with Candida albicans, PBMCs produced high levels of IL-17 and IFN-γ, CsA inhibited IL-17 and IFN-γ production in a dose dependent manner. In addition, CsA suppressed IL-17 and IFN-γ production at the early and late stages of cultures. Futhemore, CsA also reduced the frequeney of IL-17-produeing cells induced by Candida albieans. Conclusion： CsA inhibits the production of IL-17 and IFN-γ induced by Candida albieans, confirming the suppressive effect of CsA for the treatment of fungus infections.

关 键 词：环孢素A(CsA) 白色念珠菌 白细胞介素17 

分 类 号：R392[医药卫生—免疫学]