HPLC法同时测定枸磺新啶片中枸橼酸喷托维林和盐酸溴己新的含量  

Determination of Pentoxyverine Citrate and Bromhexine Hydrochloride in Pentoxyverine Citrate Sulfamethoxazole Bromhexine Hydrochloride and Thinethoporim Tablets by HPLC

作　　者：杨宏伟[1] 张迪[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《中国药事》2009年第9期914-916,共3页Chinese Pharmaceutical Affairs

摘　　要：目的建立HPLC法测定枸磺新啶片中枸橼酸喷托维林和盐酸溴己新含量的测定方法。方法采用Diamonsil C18柱;以甲醇-戊烷磺酸钠磷酸盐缓冲液（1.45g戊烷磺酸钠、3.45g磷酸二氢铵溶于900mL水中,用磷酸调pH至3.0）（55∶45）为流动相;流速为1.0mL.min-1;检测波长为220nm。结果枸橼酸喷托维林和盐酸溴己新的线性范围分别为0.26~2.33μg（r=0.9999）和0.16~1.45μg（r=0.9999）,平均回收率分别为99.8%（RSD=0.62%,n=9）和99.4%（RSD=0.79%,n=9）。结论该方法简便、快速,专属性强,可有效地控制枸磺新啶片中枸橼酸喷托维林和盐酸溴己新的含量。Objective A method for determination of pentoxyverine citrate and bromhexine hydrochloride in Pentoxyverine Citrate Sulfamethoxazole Bromhexine Hydrochloride and Thinethoporim Tablets by HPLC was established. Methods A Diamonsil C18 column was used with the mobile phase of methanol--sodium hexanesulfonate phosphate buffer solution（containing 1.45g sodium pentanesulfonate, 3.45g ammonium dihydrogen phosphate and 900mL water, adjusting to pH 3.0 by phosphoric acid） （55 ： 45）,with flow rate 1mL · min^-1 and detection wavelength at 220nm. Results A better linearity and good correlation coefficient were obtained. Pentoxyverine citrate was 0. 26 - 2. 33μg （r= 0. 9999）. Bromhexine hydrochloride was 0.16 - 1.45μg （r = 0. 9999）. The satisfactory recoveries was obtained. Pentoxyverine citrate was 99.8% （RSD= 0. 62%）. Bromhexine hydrochloride was 99.4% （RSD= 0. 79%）. Conclusions The method is convenient and accuate. It can be used as a method of quality control.

关 键 词：HPLC 枸磺新啶片 盐酸溴己新 枸橼酸喷托维林 含量测定 

分 类 号：R927.2[医药卫生—药学]