褪黑素测定方法及玉米、水稻种子中褪黑素含量的分析研究  

Study on Analysis Method of Melatonin and Melatonin Content in Corn & Rice Seeds

作　　者：王金英[1] 江川[1] 李书柯[1] 郑金贵 

机构地区：[1]福建省农业科学院水稻研究所,福州350018 [2]福建农林大学,福州350002

出　　处：《中国农学通报》2009年第17期20-24,共5页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划"水稻基因资源发掘与种质创新利用研究"(2006BAD13B01-19);农业部保种项目"野生稻考察收集;繁殖更新与分发--福建野生稻调查;自交系及导入系的构建"(NB07-070401-28-2-1)

摘　　要：褪黑素(melatonin,MT)是一种吲哚类色胺(N-乙酰-5-甲氧基色胺),MT被证实广泛存在于生物有机体中。利用玉米、水稻种子作为材料,探索种子中MT含量的测定方法、并初步了解玉米和水稻种子中MT含量情况。结果表明:用高效液相色谱法(HPLC)来检测种子中MT含量是可行的,褪黑素在0.01～1.20mg/kg浓度范围内,其标准曲线的回归方程为Y=643436X-2446.1,R2=0.9998,平均回收率达94.11%;玉米、水稻两种粮食作物种子中含有褪黑素成份,且不同种间、品种间MT含量差异很大。玉米品种MT平均含量为96.5g/kg,变幅为0～2034μg/kg;水稻平均为16.0μg/kg,变幅为0～264μg/kg。Melatonin（MT for short） is an indole-trytamine（N-Acetyl-5-hydroxy tryptamine） . It was verified that MT exists almost all biological organism. The seeds of corn and rice were utilized as the materials of the experiment,study on analysis method of melatonin and MT content in seeds of corn,rice. The results showed that：It was practicable to detect MT content in plant seeds by the method of HPLC. The regression equation was Y=643436X-2446.1 and R2=0.9998,when the concentration of melatonin was 0.01-1.20 mg/kg. The average recovery rate was 94.11%. It was indicated that MT exists in the seeds of corn and rice,and the MT concentration of various species and varieties was significant different. Average MT content of corn seed was 96.5 μg/kg,its range was 0-2034 μg/kg;Average MT content of rice seed was 16 μg/kg,its range was 0-264 μg/kg.

关 键 词：高效液相色谱法(HPLC) 褪黑素 玉米 水稻 

分 类 号：S511.02[农业科学—作物学] S513