生防菌株TL2的菌剂研制及大田防病试验  

Microbial Inoculum of Biocontrol Strain TL2 and its Effect of Proventing Diseases in Field

作　　者：洪永聪[1] 

机构地区：[1]青岛农业大学茶叶研究所,山东青岛266109

出　　处：《中国农学通报》2009年第17期181-185,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目"杀虫防病工程内生细菌植物疫苗研究"(No.30370968)

摘　　要：以一株能在茶树体内内生定殖、对茶叶斑病防病效果好的枯草芽孢杆菌(Bacillus subtilis)菌株TL2为研究对象,优化该菌株的发酵条件和生产工艺,筛选它的适宜助剂,测定它在大田对茶叶斑病的防病效果。结果表明:该菌株的最佳产朊条件为氮源(蛋白胨)、培养温度(35℃)、碳源(蔗糖)、培养时间(72h)、初始pH6.6和250ml装液量(50ml)。它的最佳固态发酵工艺为氮源(豆饼粉)、碳源(麸皮)、含水量(25ml)、接种量(4%)、培养温度(35℃)和初始pH7.0。筛选得到该菌剂的4种良好载体,碳酸钙、高岭土、滑石粉和硅藻土等,1种良好的润湿分散剂,木质素磺酸钙,以及2种优良的紫外保护剂,腐植酸和糊精。该菌剂(孢子粉可湿性粉剂)在田间对茶叶斑病有较好的防治效果,防效达70%以上,与对照药剂多菌灵的防治效果相当。Bacillus subtilis strain TL2 was studied,which was proved to be colonize inside of tea plant and had good effect of preventing tea leave spot diseases.The fermentation conditions,processing technique,accessory ingredient and control effect against leave spot diseases in field of this strain were tested.The result indicated that its optimum conditions of producing antimicrobial protein should be nitrogen source（peptone）,cultivation temperature（35 ℃）,carbon source（sucrose）,cultivation time（72 h）,original pH 6.6 and liquid medium volume（50 ml/250ml）.Its optimum conditions of solid state fermentation was tested to be nitrogen source（defatted soybean）,carbon source（bran）,moisture content（25 ml/250ml）,inoculum concentration（4%）,cultivation temperature（35 ℃） and original pH 6.6.Four kinds of accessory ingredient（calcium carbonate,kaoline,talcum powder and kieselguhr）,one kind of wetting dispersants（calcium lignosulphonate） and two kinds of protective agent from UV（humic acid and dextrine）,were screened.The microbial inoculum（wettable powder） was tested to have good effect of preventing tea leave spot diseases（70%） in tea garden,which was appropriate to the effect of Sanmate as a control.

关 键 词：生防菌 枯草芽孢杆菌 茶叶斑病 菌剂 防病效果 

分 类 号：S435.711[农业科学—农业昆虫与害虫防治]