漆酶体系酶解马尾松浆表面性能的研究  

Study on Surface Characteristics of the Masson Pine Pulp Enzymelyzed with Three Laccase Systems

作　　者：尤纪雪[1] 王玉秀[1] 

机构地区：[1]南京林业大学化学工程学院,南京林业大学江苏省制浆造纸科学与技术重点实验室,江苏南京210037

出　　处：《中国造纸学报》2009年第3期37-41,共5页Transactions of China Pulp and Paper

基　　金：国家自然科学基金资助项目(30671654)

摘　　要：对漆酶/介体体系(LMS)、由白腐菌直接合成的漆酶/木聚糖酶体系(LXS)以及复配的漆酶/木聚糖酶体系(L+X)3种漆酶体系降解木质素的能力进行比较,并应用扫描电镜及X射线光电子能谱(XPS)表面分析等手段从微观角度探讨了酶处理降解木质素的机理。结果表明,L+X降解木质素的能力不如LXS和LMS,若用L+X取代LMS并不能达到理想的处理效果。通过扫描电镜观察及浆料的XPS表面分析,进一步证明了LXS具有与LMS相似的木质素降解能力,用LXS完全可以代替LMS用于生物制浆和生物漂白等领域。The delignification abilities of laccase/mediator system （LMS）, laccase/xylanase system （LXS） from white rot fungus and composite laccase/xylanase （ L ＋ X） were compared in this paper. The mechanisms of lignin degradation with three enzyme systems treatment were studied by the analysis of X-ray photoelectron spectroscopy （XPS） and Scanning electron microscope （SEM）. The results indicated that the delignification ability of L ＋ X is not as good as LXS and LMS. The SEM observation and surface analysis of the pulp further illustrated that LXS has as strong delignification ability as LMS. The LXS can entirely replace expensive and complicated LMS for bio-delignification application.

关 键 词：漆酶/木聚糖酶体系 漆酶/介体体系 木质素脱除 XPS分析 

分 类 号：TS743.14[轻工技术与工程—制浆造纸工程]