猪LXRα基因的克隆、序列分析及表达研究  

Cloning,Sequence and Express Patterns Analysis of Porcine LXRα Gene

作　　者：于浩[1] 刘娣 丁镌[4] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]黑龙江省农科院,哈尔滨150086 [3]东北农业大学动物科技学院,哈尔滨150030 [4]黑龙江省血液中心,哈尔滨150056

出　　处：《中国农学通报》2009年第18期18-21,共4页Chinese Agricultural Science Bulletin

摘　　要：采用RT-PCR结合克隆测序的方法,从猪的肝脏组织中克隆出了猪基因的LXRα(Liver XReceptorsα)cDNA序列,编码区长度1344bp,编码447个氨基酸,基于Pfam数据库发现存在一个锌指蛋白和核受体的配体结合区;系统发育分析发现猪的LXRα所在的哺乳动物类群非常保守,与鸡和斑马鱼所构成的类群存在较大遗传距离;在大白猪和杂种野猪的脂肪组织中检测表明LXRα基因的表达自3月龄至12月龄逐渐增加,并在9月龄达到最高,而杂种野猪在9月龄开始高表达,12月龄最高。研究结果表明LXRα基因可能是猪脂质代谢的重要分子标识之一。Cloning and sequencing the LXRα（Liver X Receptor α） cDNA from porcine liver tissues by RT-PCR, the coding region length was 1344bp, coding 447 Amino Acids, Zinc finger and ligand-binding domain of nuclear hormone receptor are found based on the Pfam database, phylogenetic result showed that the LXRα genes in Mammalia clade were very conserved, the genetic distance between the Mammalia clade and non-Mammalia clade including chicken and zebrafish, LXRα mRNA level were detected in the adipose tissues showed that LXRα mRNA expression increased from 3-month to 12-month, the mRNA level was the most high in 9-month in large white pig, but the LXRα mRNA expression were detected in 9 month, and the expression in 12-month was high in crossed-bred wild pig. The results showed that LXRot gene maybe one of important molecular markers in lipid metabolism of pig.

关 键 词：猪 LXRΑ RT-PCR 系统发育分析 

分 类 号：S828[农业科学—畜牧学]