SRAP构建玉米杂交种指纹图谱的研究  

Development of SRAP Fingerprinting in 19 Maize Hybrids

作　　者：黄进勇[1] 盖树鹏[1] 张恩盈[1] 夏连胜[1] 

机构地区：[1]青岛农业大学生命科学学院,山东青岛266109

出　　处：《中国农学通报》2009年第18期47-51,共5页Chinese Agricultural Science Bulletin

基　　金：山东省中青年科学家奖励基金项目"玉米品种(系)分子鉴定系统研制"(2004bsb01404)

摘　　要：SRAP(相关序列扩增多态性)是近年来发展起来的一种新型的DNA标记技术,作者旨在探讨SRAP技术用于玉米品种鉴定的可行性,并建立相应技术体系。采用25个引物组合对19个玉米杂交种进行了SRAP扩增。19个引物组合扩增出了多态性条带,共扩增出152条多态性条带,平均每个引物组合产生8条多态性带,多态性频率从44.4%(me4/em3)～91.7%(me4/em1)。19个引物组合检测到的平均PIC为0.879,而引物组合me4/em1可区分供试的所有19个玉米品种。进一步筛选了5对引物,构建了供试材料的指纹图谱,为SRAP用于玉米鉴定奠定了基础。SRAP具有很高的分辨率,而且操作简便、稳定可靠,具备了用于作物品种鉴定的条件,用于玉米品种鉴定是可行的,可作为SSR技术的重要补充。Sequence-related amplified Polymorphism（SRAP） is a fairly new PCR-based molecular marker technique developed recent years. The objective of this study was to discuss the possibility of applying SRAP in maize hybrid identification, and to develop identification system.Nineteen maize hybrids were analyzed by SRAP technique with 25 pairs of SRAP primers. Nineteen pairs out of 25 pairs of SRAP primers were selected, which displayed polymorphism, stable and repeated amplification profiles. A total of 152 polymorphic SRAP bands were detected and one primer pair produced 8 polymorphic bands on average. The ratio of poly- morphic bands was from 44.4% （me4/em3）to 91.7%（me4/em1）. The Polymorphic Information Content （PIC） for the SRAP varied from 0.752 to 0.922 with an average of 0.879. Prominently, primer pair me4/eml can dis- tinguish the 19 maize hybrids by itself. Furthermore, 5 primer pairs were selected and fingerprinting of 19 maize hybrids were developed, which would be basement of maize identification by SRAP technique.The resuits verified very high discrimination ability of SRAP markers, moreover, considering its simple, stable, co-dominant and well- proportioned distribution, conclusion could be made that SRAP might be a potentially useful marker technique and supplement for SSR in maize hybrid identification.

关 键 词：玉米杂交种 SRAP 品种鉴定 指纹图谱 

分 类 号：S513[农业科学—作物学]