橡胶愈伤组织超低温保存的研究  

Study on Callus Cryopreservation of Hevea brasiliensis Muell. Arg

作　　者：赵平娟[1] 崔百明[1,2] 孙海彦[1] 彭明[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,海口571101 [2]石河子大学生命科学学院,新疆石河子832003

出　　处：《中国农学通报》2009年第18期413-416,共4页Chinese Agricultural Science Bulletin

基　　金：2008年海南省高校博士研究生创新科研课题立项项目(Hxwby2008-02);海南省自然科学基金项目(30822);中央级公益性科研院所基本科研业务费项目(ITBBZD0711)

摘　　要：为研究长期有效的保存橡胶种质资源新方法,以解决传统橡胶种质资源大田保存方式花费大量的人力、物力和土地,不利于橡胶种质资源在国际间的交换,制约了橡胶树杂交育种工作进展的问题。选用海南广泛栽培的7-33-97橡胶花药脱分化和松散型胚性两种不同形态的愈伤组织经低温驯化、脱水处理、低温保存、解冻处理后,在橡胶愈伤组织增殖培养基上培养,比较不同处理间愈伤组织恢复增殖能力的差别。结果表明:常温培养的松散型胚性愈伤组织,经过-80℃先慢降温再-196℃快降温,液氮保存,在40℃水浴解冻后容易恢复增殖能力。因此,采用先慢后快的降温方式,40℃的解冻方式保存松散的胚性愈伤组织是最优的橡胶愈伤组织保存方法。可以采用这种方法对现有多个橡胶无性系愈伤组织进行保存。Plantations was a basic way of rubber germplasm resources preservation, however, it needed take a lot of manpower, material and land. Callus cryopreservation was an ideal method of long-term preservation germplasm and the method can promote international exchange of germplasm. The two kinds of callus induced from rubber anther were acclimated, dehydrated, preserved, thawed and then cultured in callus induction culture medium. The results showed that friable callus was easily restored the ability of proliferation, cooling at -80℃, preserving at 196℃ and thawed at 40℃ was the better way to the same callus. So friable callus adapt to cooling way from fast to slow and thawing way in 40℃ was the best method of rubber callus preservation. The method may be applied in several rubber clones.

关 键 词：橡胶树 愈伤组织 超低温保存 

分 类 号：S666.035[农业科学—果树学]