霍乱弧菌O1群菌株多位点酶电泳分析  

Analysis of Vibrio cholerae O1 with the method of multilocus enzyme electrophoresis

作　　者：段广才[1] 高守一[2] 刘延清[2] 祁国明[2] 章丽娟[2] 

机构地区：[1]河南医科大学公共卫生学院流行病学教研室 [2]中国预防医学科学院流行病学微生物学研究所

出　　处：《河南医科大学学报》1998年第3期11-17,共7页Journal of Henan Medical University

基　　金：国家自然科学基金

摘　　要：为更准确区分霍乱弧菌Ｏ１群不同菌株的流行病学意义，应用多位点酶早泳法对来自不同国家，地区，时间和人群的霍乱弧菌Ｏ１群菌４７１株进行分型和分子生物学数值分类分析。结果；通过１１种酶基因位点的检测，４７１株Ｏ１群霍乱弧菌可以分为７１个ＥＴ型，ＥＴ１－ＥＴ２８为噬菌体－生物分型所区分的流行株，其中ＥＴ１－ＥＴ６为古典型菌，ＥＴ７包括大多埃尔托流行株和部分古典型菌株；Cholera, as an acute infectious disease, is one of the serious problems of public health in the developing countries. The strains of Vibrio cholerae serogroup O1,classified by the biotyping and serotyping method, have been recognized the pathogen of epidemic cholera. In order to recognize the epidemiological significance of different isolates of Vibrio cholerae serogroup O1, 471 strains of Vibrio cholerae O1 isolated from China and other countries were analyzed with multilocus enzyme electrophoretic (MEE) method depending on 11 enzyme loci. 71 ETs were obtained, and all of the epidemigenic strains of Vibrio cholerae O1 classified by phage biotyping method included in ET1～ET28. Vibrio cholerae classical belonged to the ET1～ET6, and ET7. The epidemigenic strains of Vibrio cholerae El Tor belonged to ET7～ET28. By the clonal analysis 12 CLs(clonal clusters) were obtained and all of the epidemigenic strains of Vibrio cholerae O1 including classical and El Tor strains belonged to CL K. The results showed that the genetic relationships among epidemigenic strains of Vibrio cholerae O1 were very close compared with non epidemigenic strains of Vibrio cholerae O1 the latter held more ETs.

关 键 词：霍乱弧菌 分型 多位点酶电泳 数值分类 O1群 

分 类 号：R378.3[医药卫生—病原生物学] R372[医药卫生—基础医学]