神经节苷脂GM_3诱导HL-60细胞分化后中性糖脂的变化  

Changes of neutral glycosphingolipid composition of differentiated human promyelocytic leukemia cell line HL 60 by gangliosids GM 3

作　　者：侯卫红[1,2,3,4] 赵卫星[1,2,3,4] 张莉蓉 张运晓[1,2,3,4] 秦国斌[1,2,3,4] 

机构地区：[1]河南医科大学生物化学教研室 [2]河南医科大学临床药理学教研室 [3]河南医科大学有机化学教研室 [4]安阳市人民医院外科

出　　处：《河南医科大学学报》1998年第3期92-94,共3页Journal of Henan Medical University

摘　　要：为了研究神经节苷脂ＧＭ３（Ｇａｎｇｌｉｏｓｉｄｅ，ＧＭ３）对人早幼粒白血病细胞系ＨＬ６０细胞诱导分化后中性糖脂的影响，将神经节苷脂ＧＭ３加到ＨＬ６０细胞的ＤＭＥ／Ｆ１２培养液中，观察其对ＨＬ６０细胞的诱导分化作用及分化后细胞中性糖脂的变化。结果：细胞吞噬功能显著增强，非特异性酯酶活性升高，细胞形态也呈现与这种分化相一致的改变。神经节苷脂ＧＭ３诱导ＨＬ６０细胞分化后细胞中性糖脂各组分的百分含量发生了显著改变，ＣＤＨ、ＣＭＨ及ＣＱＨ显著增多，与分化前相比Ｐ＜００５；ＣＸＨ显著降低，与分化前相比Ｐ＜００５。The purpose of the present study was to investigate the changes of the neutral glycosphingolipid composition of a human promyelocytic leukemia cell line HL 60 cells differentiated by gangliside GM 3, After HL 60 cells were cultured with exogenous GM 3 in DME/F12 medium,that the effects on differentiation and change of neutral glycosphingolipid composition of HL 60 cells were observed.Results:The percentage of phagocytosing and the non specific esterase(NSE) activity significantly increased.Cells showed morphologically that they were differentiated into monocyte macrophage cells.After HL 60 cells were differentiated by gangliside GM 3,the remarkable increase was CDH、CMH and CQH( P <0 05 compared with undifferentiated),CXH showed significant decrease( P <0 05 compared with undifferentiated).It is suggested that Gangliside GM 3 can change neutral glycosphingolipid composition of the HL 60 cells.

关 键 词：神经节苷脂 HL-60细胞 中性糖脂 白血病 

分 类 号：R392－12[医药卫生—免疫学] R733.7[医药卫生—基础医学]