快速定量检测试剂盒测定尿碘的方法验证  

Validation of rapid test kit method for measuring iodine in urine

作　　者：万琛[1] 龚燕[1] 宋振威[1] 

机构地区：[1]江苏省无锡市疾病预防控制中心,江苏无锡214023

出　　处：《中国卫生检验杂志》2009年第9期2002-2003,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:本文对尿碘快速定量检测方法进行方法验证,并且通过与国标法的测定结果比对,验证快速定量检测试剂盒方法的灵敏度、准确度与精确度。方法:尿碘快速定量检测方法及国标法。结果:快速法最小检出下限为7.5μg/L,分别对碘含量为64.7、138、231μg/L的尿碘标准物质进行6批测定,与标准值的偏差为0.85%,0.83%,0.65%;变异系数分别为3.6%,1.9%,1.4%。540份现场样品全部用试剂盒法进行检测,并抽取100份与国标法进行对比测定,测定结果的差异无显著意义。结论:快速法为一种简易、快速、准确的尿碘测定方法。Objective：To validate the rapid test kit method and compare the result with the GB standard. It determined the rapid test method＇s resolution and accuracy. Methods： Rapid test kit method for measuring iodine in urine and GB standard. Results： The low detection limit of rapid test method is 7.5 μg/L. 3 different Iodine standard samples （64. 7,138,231 μg/L） were repeated for 6 times. All 540 samples were tested by rapid test kit method and 100 of them were compared with GB standards. The standard deviation is 0. 85% ,0. 83% ,0. 65% and RSD is 3.6%, 1. 9%, 1.4%. The experiment showed there is no significant difference between rapid test method and GB standards. Conclusion： Rapid test kid method is simple, fast and accurate for measuring iodine in urine.

关 键 词：快速试剂盒法 国标法 尿碘 

分 类 号：R446.12[医药卫生—诊断学]