献血者中TT病毒DNA及IgG抗体检测结果的探讨  

Detection of TT virus DNA and anti-TTV IgG in sera of blood donors

作　　者：田明丽 杨爱玲 戚晓东 

机构地区：[1]山东省威海市中心血站,山东威海264200

出　　处：《中国卫生检验杂志》2009年第9期2064-2066,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:了解献血者中输血传播病毒(TTV)的感染状况。方法:应用套式PCR(nested-PCR)和ELISA方法同步检测了388份献血者、168份非肝炎住院患者(对照组)的血清TTV DNA和抗TTV IgG。结果:TTV基因检出率分别为献血者15.46%(ALT正常组13.37%,ALT异常组41.38%,P<0.05),对照组24.40%(ALT正常组23.78%,ALT异常组50.00%,P<0.05);556份受检血清中TTV DNA检出率为18.17%,ALT正常组16.60%,ALT异常组42.31%,P<0.05两者存在显著性差异。抗TTV IgG检出率分别为献血者13.14%(ALT正常组11.70%,ALT异常组31.03%,两者存在显著性差异,P<0.05),对照组为27.98%(ALT正常组26.83%,ALT异常组75%,P<0.05),献血者组与对照组相比TTV DNA及抗TTV IgG均存在显著性差异(P<0.05)。结论:献血者组和对照组中存在TTV感染,TTV感染与ALT异常有关,提示TTV可能有一定的致病性。TTV感染和输血安全性有待进一步研究。抗TTV IgG可用于检测TTV感染。Objective：To investigate the prevalence of TT virus （TTV） in blood donors. Methods：TTV DNA and anti -TTV IgG were simultaneously detected by nested - PCR and ELISA. Results： The positive rate of TFV DNA in the samples from blood donors was 15.46% （60/388）. Blood donors with normal transaminase levels of 13.37% （48/359） and those with elevated transaminase levels of 41.38% （12/29） were positive for TTV DNA in samples from the non - hapatitis patients （ control group） was 24.40% （41/168）, those with normal transaminase levels of 23. 78% （39/164）and those with elevated transaminase levels of 50. 00% were positive rate for TFV DNA, there was significant difference between them （ P 〈 0. 05 ）. The positive rate of rTTV DNA in all of samples was 18. 17% （101/556）. The samples with normal transaminase levels of 16. 60% （88/530） and the sampies with elevated transaminase levels of 42. 31% （11/26） were positive for TTV DNA, there was significant difference between them （ P 〈 0. 05 ）. The positive rate of anti - TTV IgG in the samples from blood donors was 13.14% （ 51/388 ）, Blood donors with normal transaminase levels of 11.70% （42/359） and those with elevated transaminase levels of 31.03 % （9/29） was positive for anti - TTV IgG, there was significant difference between them （ P 〈 0. 05 ）. The positive rate for anti - TTV IgG in the samples of control group was 27. 98% （47/168）, they with normal transaminase levels of 26. 83% （44/164） and those with elevated transaminase levels of 75. 00% （ 3/4 ） were positive for anti - TTV IgG, there was significant difference between them （ P 〈 0. 05 ）. there was no significant difference between positive rate for TFV DNA and anti - TTV IgG. Conclusion：The investigation suggested that there exsits TTV infect in blood donors and control group. TFV infection may be related to abnormal ALT and as pathogen. The relationship between TTV infection and security of blood transfusion need to study fur

关 键 词：TT病毒 献血者 套式聚合酶链反应 IGG 

分 类 号：R446.11[医药卫生—诊断学]