猪乙型脑炎病毒E基因的克隆与序列分析  

Cloning and Sequence Analysis of E Gene of Japanese Encephalitis Virus

作　　者：丛晓燕[1] 吴家强[1] 王怀忠[1] 徐文[2] 张秀美[1] 王金宝[1] 陈溥言[3] 

机构地区：[1]山东省农业科学院畜牧兽医研究所,济南250100 [2]青岛农业大学,青岛266109 [3]南京农业大学动物医学院,南京210095

出　　处：《中国畜牧兽医》2009年第9期71-73,共3页China Animal Husbandry & Veterinary Medicine

基　　金：农业公益性行业科研专项(200803015)

摘　　要：根据GenBank中猪乙型脑炎病毒SA14-14-2基因序列设计2对引物,从分离的猪源乙型脑炎病毒SD-001株的细胞培养物中扩增出包括E基因全长的两段基因,将扩增的目的片段进行克隆与序列分析。结果表明,所克隆的E基因编码结构域(Domain)区段与SA14-14-2、P3、Beijing-1等毒株的核苷酸与氨基酸序列同源性分别达97.2%与96.8%以上,属于Ⅲ型乙型脑炎病毒,与疫苗株SA14-14-2的Domain区相比,共有8个氨基酸位点变异。According to the published E genes sequence of JEV in GenBank, two pairs of PCR primers were designed. E genes were amplified from JEV-SD001 strain. Then the PCR products were cloned into pMD-18 Easy Vector, and the recombinant plasmids were obtained. The sequence of the cloned PCR products were analyzed with other JEV isolates in GenBank. The results indicated that the homology of their nucleotide sequence was over 97.2%, and it shared over 96.8% of amino acid homology with other JEV isolates. JEV-SD001 belonged to Japanese encephalitis virus genotype Ⅲ, E protein of isolates showed some differences as compared with vaccine strains.

关 键 词：猪乙型脑炎病毒 E基因 序列分析 

分 类 号：Q78[生物学—分子生物学]