间接免疫细胞化学法快速检测新城疫病毒抗原的研究  

Detection of NDV Infection and Antigen Location by Indirect Immunocytochemistry

作　　者：彭明义[1] 程宝艳[2] 戴银[1] 程帮照[1] 张春玲[1] 余为一[1] 

机构地区：[1]安徽农业大学动物科技学院,合肥230036 [2]安徽省农业科学院畜牧兽医研究所,合肥230031

出　　处：《中国畜牧兽医》2009年第9期126-130,共5页China Animal Husbandry & Veterinary Medicine

基　　金：安徽省"十五"科技攻关项目(01013003)资助

摘　　要：本研究旨在建立一种简单、方便、准确、特异的方法检测新城疫病毒(Newcastle disease virus,NDV)。比较间接HRP-免疫细胞化学法与间接免疫荧光法两种方法检测NDV在COS-7细胞中的定位。两种试验方法都可以检测到NDV主要定位在COS-7细胞的胞浆内。间接HRP-免疫细胞化学法检测NDV要求一抗滴度≤1∶100,间接免疫荧光法要求的滴度≤1∶100。间接HRP-免疫细胞化学法检测NDV要求二抗滴度≤1∶300,间接免疫荧光法要求的滴度≤1∶50。而且结果观察中间接HRP-免疫细胞化学法要比间接免疫荧光法方便。间接HRP-免疫细胞化学法进行NDV检测优于间接免疫荧光法,是一种简单、快捷、准确的适用于检测NDV的技术。The objective is to establish a simple, convenient, accurate, specific method to detect the NDV and detect the location of NDV in the COS-7 by comparing the indirect HRP-immunocytochemistry and indirect fluorescence immunocytochemistry. Both two experiment methods can detect the location of NDV and it was in the cell plasm of the COS-7. The indirect HRP-immunoeytochemistry request the dilution of the first antibody is less than 1 ： 100, but the indirect fluorescence immunocytochemistry request the dilution of the first antibody is less than 1 ： 100. The indirect HRP-immunoeytoehemistry request the dilution of the second antibody is less than 1 ： 300, but the indirect fluorescence immunocytochemistry request the dilution of the second antibody is less than 1 ： 50. Further more, when detect the results that the indirect HRP-immunoeytoehemistry is more convenient than the indirect fluorescence immunocytoehemistry. The indirect HRP-immunoeytoehemistry is simple, convenient, accurate and specific method to detect the NDV.

关 键 词：免疫酶细胞化学法 荧光细胞化学法 间接 新城疫病毒 抗原定位 

分 类 号：S854.43[农业科学—临床兽医学]