不同光照下褪黑素变化对鸡骨髓内c-fos原癌基因表达的影响  

Effects of Melatonin on c-fos Oncogene Expression of the Bone Marrow of Chicken in Different Illumination Conditions

作　　者：宋丽霞[1] 刘淑英[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院,呼和浩特010018

出　　处：《中国畜牧兽医》2009年第9期186-189,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：为了找出褪黑素分泌变化与c-fos原癌基因表达之间的关系,本研究控制不同光照时间饲养1日龄雏鸡50只,取骨髓组织,经免疫组化试验,结果显示:①单核细胞系胞浆出现阳性信号;巨核细胞系、粒细胞系和淋巴细胞系胞核出现阳性信号;红细胞系无阳性信号;②随着光照时间的延长,c-fos原癌基因的表达量呈增长趋势。结果表明,c-fos与骨髓细胞的细胞增殖和分化相关。褪黑素的增高能够显著抑制c-fos原癌基因的表达,且褪黑素的减少能够刺激c-fos的表达。In order to find out the relationship between the changes of melatonin secretion and expression of c-fos oncogene, in this study, we have one day-old chicks by controlling different illumination conditions, then take out 1 cm bone marrow, immunohistochemical analysis showed that： （1）positive signal in the cytoplasm of monocytic series; positive signal in the cell nuclei of megakaryocytic series, granulocytic series and lymphocyteic series; no positive signal in erythrocyte. （2）With the extension of illumination time, the expression of c-fos had a growth trend. The results showed that c-fos is related to cell proliferation and differentiation of bone marrow cells. The increase of melatonin can significantly inhibit c-fos oncogene expression and the reduction of melatonin can stimulate c-fos expression. According to the results of this experiment can speculate that melatonin can impact on proliferation and differentiation of bone marrow cells by regulating the expression of c-fos.

关 键 词：褪黑素 C-FOS原癌基因 骨髓 

分 类 号：S852[农业科学—基础兽医学]