高效液相色谱-电感耦合等离子体质谱联用技术测定二价汞、甲基汞、乙基汞与苯基汞  

DETERMINATION OF INORGANIC MERCURY,METHYL-MERCURY,ETHYL-MERCURY AND PHENYL-MERCURY BY HPLC-ICP-MS

作　　者：张兰[1] 陈玉红[2] 施燕支[1] 王英锋[1] 米健秋[2] 张之旭[2] 

机构地区：[1]首都师范大学分析测试中心,北京100048 [2]安捷伦科技(中国)有限公司,北京100102

出　　处：《环境化学》2009年第5期772-775,共4页Environmental Chemistry

摘　　要：建立了高效液相色谱-电感耦合等离子体质谱联用技术(HPLC-ICP-MS)测定二价汞、甲基汞、乙基汞、苯基汞等4种汞形态的分析方法.该方法采用醋酸铵/L-半胱氨酸缓冲盐及甲醇体系组成的流动相按一定比例进行梯度洗脱,并结合了ICP-MS的时间分辨采集优化程序.二价汞、甲基汞、乙基汞、苯基汞的检出限分别为0.022,0.022,0.028和0.041ng.ml-1.对5.0ng·ml-1的混合标准溶液连续测定6次,4种汞化合物的峰面积相对标准偏差(RSD)分别为1.40%,1.01%,0.97%和2.13%.A method was established for the determination of inorganic mercury, methyl-mercury, ethyl-mercury and phenyl-mercury by high performance liquid chromatography coupled with inductively coupled plasma-mass spectrometry （HPLC-ICP-MS）. Ammonium acetate/L-cysteine/methanol were used as mobile phase for the separation of inorganic mercury, methyl-mercury, ethyl-mercury and phenyl-mercury by the gradient elution. The detect limits were 0. 022 ng· ml^-1 for inorganic mercury, 0. 022 ng·ml^-1 for methyl-mercury, 0. 028 ng·ml^-1 for ethyl-mercury and 0. 041 ng·ml^-1 for phenyl-mercury respectively. The relative standard deviations for peak area of 5.0 ng · ml^-1 mixed standard solution were： 1.40% for inorganic mercury, 1.01% for methyl-mercury, 0. 97% for ethyl-mercury, 2. 13% for phenyl-mercury （n=6） .

关 键 词：HPLC—ICPMS 二价汞 甲基汞 乙基汞 苯基汞. 

分 类 号：X830[环境科学与工程—环境工程]