低氧对AtT-20细胞HIF-α、GR和POMC基因表达的影响及意义  

Effect of hypoxia on the expressions of hypoxia-inducible factor-lot, glucocorticoid receptor and opiomelanocortin genes in AtT-20 cells

作　　者：张晨冉[1] 孙青芳[1] 卞留贯[1] 赵卫国[1] 沈建康[1] 李娜[2] 李小英[1] 宁光[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院神经外科,上海200025 [2]上海市内分泌代谢病临床医学中心上海市内分泌代谢病研究所,上海200025

出　　处：《中华神经医学杂志》2009年第9期873-876,共4页Chinese Journal of Neuromedicine

基　　金：基金项目：上海市自然科学基金（08ZR1413800）

摘　　要：目的探讨氯化钴（CoCl2）对小鼠促肾上腺皮质激素（ACTH）垂体腺瘤细胞AtT-20中低氧诱导因子-1α（HIF-1α）、糖皮质激素受体（GR）和阿片黑素促皮质激素原（POMC）表达的影响及其意义。方法25、50、100、200μmol／LCoCl2处理AtT-20细胞诱导其发生低氧，MTT检测细胞增殖的变化，实时定量PCR和Westernblot分别检测HIF-1α、GR、POMCmRNA和蛋白的表达情况。结果与对照组比较，25、50、100μmol／LCoCl2处理AtT-20细胞24、48h均可促进细胞生长增殖，处理AtT-20细胞24h其HIF—hx、GR、POMCmRNA和蛋白表达水平增高，且呈浓度依赖性，差异有统计学意义伊〈0．05）。结论低氧可诱导ArT-20细胞中H1F-1α，GR和POMCmRNA和蛋白表达水平的增加。Objective To investigate the effect ofCoC12-induced hypoxia on the expressions of hypoxia-inducible factor-1α （HIF-1α）, glucocorticoid receptor （OR） and opiomelanocortin （POMC） in ART-20 cells. Methods ART-20 ceils were exposed to different concentrations （25, 50, 100, and 200 μmol/L） of COCl2 to induce hypoxia. MTT assay was employed to assess the changes in the cell proliferation after the exposure, and real-time PCR and Western blotting were performed to detect the expressions of HIF-1α, GR and POMC genes at both the mRNA and protein levels. Results Compared with the cells in normoxic conditions, the AtT-20 cells exposed to COC12 treatment at 25, 50, and 100 μmol/L for 24 and 48 h showed obviously enhanced cell proliferation. COCl2 significantly increased the mRNA and protein expressions of HIF-1α, OR and POMC genes in a dose-dependent manner （P〈0.05）. Conclusion CoCl2-induced hypoxia up-regulates the mRNA and protein expressions ofHIF-1α GR and POMC genes in AtT-20 cells.

关 键 词：低氧诱导因子-1Α 糖皮质激素受体 阿片黑素促皮质激素原 

分 类 号：R686[医药卫生—骨科学]