香烟烟雾对雄性大鼠性功能影响的实验研究  

Cigarette Smoke Affects Sexual Function of Male Rats

作　　者：占习双[1] 吴天鹏[1] 欧阳海[1] 刘小兵[1] 蔡惠丽[2] 

机构地区：[1]武汉大学人民医院泌尿外科,湖北武汉430060 [2]武汉大学中南医院血液科,湖北武汉430060

出　　处：《中华男科学杂志》2009年第9期788-791,共4页National Journal of Andrology

基　　金：湖北省教育厅重点资助项目(2008CDB209)~~

摘　　要：目的:研究香烟烟雾对雄性大鼠性功能的影响。方法:30只SD雄性大鼠随机均分为两组,吸烟组制备大鼠吸烟模型,使用市售红金龙过滤咀香烟(每支焦油含量13mg),吸烟组每日使其被动吸烟4次,每次两支,每次30min持续60d;对照组正常饲养。实验结束前1周两组动物笼中分别放入5只雌性大鼠,采用24h监控录像监测观察骑跨动作1周,60d后处死取标本。测定大鼠血中睾酮水平及阴茎海绵体组织一氧化氮合酶(NOS)的活性,行阴茎海绵体组织的Masson染色图像分析。结果:吸烟组大鼠骑跨动作次数减少,血中睾酮水平明显降低(P<0.05),阴茎海绵体组织NOS活性及平滑肌面积显著降低(P<0.05),胶原纤维明显增加,海绵窦血管腔有明显变化。结论:吸烟组大鼠阴茎勃起功能受到明显影响,睾酮水平降低及阴茎海绵体组织NOS活性降低、阴茎海绵体平滑肌面积的减少可能是其重要的发病机制。Objective： To study the effect of cigarette smoke on the sexual function of male rats. Methods ： Based on Ozyurt＇s smoking model, we equally divided 30 male adult Sprague-Dawley rats into a control and a smoking group, and exposed the latter to cigarette smoke for 60 days. A week before the end of the experiment, we added 5 female rats to each group and observed their mating through 24-hour video surveillance. Sixty days later, all the rats were killed for the determination of the level of testosterone （T） in the plasma and the activity of nitric oxide synthase （NOS） in the corpus cavernosum, and Masson-dyeing image analysis of the penile tissue. Results ： Compared with the controls, the rats in the smoking group showed a significant reduction in the times of mating, the level of plasma T （ P 〈 0.05 ） and the activity of NOS in the penile cavernous tissue （ P 〈 0.05 ）, but a slight increase in the collagen fibers and obvious changes in the blood sinuses. Conclusion： Cigarette smoke seriously affected penile erection in the experimental rats. The decrease in plasma T, NOS activity and the area of smooth muscle may be an important mechanism underlying their erectile dysfunction.

关 键 词：吸烟 勃起功能障碍 睾酮 阴茎海绵体平滑肌 一氧化氮合酶 

分 类 号：R698.1[医药卫生—泌尿科学]