双氢青蒿素和阿奇霉素对小鼠体内弓形虫速殖子超微结构的影响  

In vivo Effect of Dihydroartemisinin and Azithromycin on the Ultrastructure of Toxoplasma gondii Tachyzoites

作　　者：尹卫东[1] 高全成 刘向东[3] 唐红炜[3] 

机构地区：[1]河北北方学院临检教研室,张家口075000 [2]邯郸峰峰矿务局总院检验科,邯郸056200 [3]河北北方学院寄生虫学教研室,张家口075000

出　　处：《中国寄生虫学与寄生虫病杂志》2009年第4期325-327,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：将30只昆明小鼠随机均分为3组,即双氢青蒿素治疗组(A组)、双氢青蒿素和阿奇霉素联合治疗组(B组),以及感染对照组(C组)。每鼠腹腔注射感染2×103个弓形虫速殖子。感染8h后,A组灌服双氢青蒿素75mg/(kg·d),间隔12h再给药1次;B组同A组,另于感染24h后灌服阿奇霉素200mg/(kg·d)1次。A、B两组均连续给药4d,双氢青蒿素间隔12h给药1次,阿奇霉素间隔24h给药1次。C组不给药。治疗后(即于感染96h后),分别随机从各组取1只小鼠解剖,抽取腹水,分离弓形虫速殖子。常规制备透射电镜标本,观察其超微结构变化。结果显示,A、B两组弓形虫速殖子均出现水肿,细胞膜结构模糊,局部断裂或破损。细胞质脂滴增多、形成空泡。而感染对照组未出现上述变化。Thirty Kunming mice were randomly divided into three groups named as dihydroartemisining roup （A） , dihydroartemisinin and azithromycin group （B） , and controlgroup （C） . Each mouse was infected intraperitoneally with 2×103 Toxoplasma gondii tachyzoites. Eight hours after infection, the mice of groups A and B were treated twice a day for 4 days with 75 mg/kg of dihydroartemisinin. At 24 hours post infection, those in group B were treated once daily for 4 days with 200 mg/kg of azithromycin. At 96 hours post infection, ascites was taken from one mouse each group and the tachyzoites were collected. The ultrastructure of tachyzoites was observed by conventional transmission electron microscopy. It was found that the tachyzoites in groups A and B showed edema and enlarged, the cell membrane became indistinct, broken or damaged; fat droplets in the cytoplasm increased, and vacuoles were formed. Similar changes were not seen in the control group.

关 键 词：双氢青蒿素 阿奇霉素 弓形虫速殖子 小鼠 超微结构 

分 类 号：R382.5[医药卫生—医学寄生虫学]