HepG2细胞膜上乙型肝炎病毒前S1蛋白(preS1)结合蛋白的研究  

Detection of Cellular Binding Protein of Hepatitis B Virus PreS1 Domain on HepG2 Cell Membane

作　　者：丁岗强[1] 张君[1] 杨凤[1] 杨键[1] 向光明[2] 唐霓[1] 黄爱龙[1] 

机构地区：[1]重庆医科大学感染性疾病分子生物学教育部重点实验室,重庆400016 [2]四川省广元市中心医院,广元628000

出　　处：《中国生物工程杂志》2009年第9期71-74,共4页China Biotechnology

基　　金：国家青年科学基金(30700707)资助项目

摘　　要：采用pull down技术研究preS1在HepG2细胞膜上的结合蛋白。以原核表达的GST-preS1融合蛋白为探针蛋白,与生物素标记的HepG2细胞裂解液进行pull down试验分离与preS1结合的膜蛋白。Western blot结果显示HepG2细胞膜上有一大小约110kDa蛋白(p110)与preS1结合。通过对比实验证明该蛋白具有较好的组织特异性和种属特异性。研究结果显示该蛋白是HepG2细胞膜上与preS1结合的蛋白,可能与HBV的早期感染过程有关。To search for novel HBV preS1 -interacting proteins on HepG2 cell membrane, preS1 protein was recombined with GST tag and GST pull down assay was employed to detection of preS1 domain cellular binding protein, using the precleared lysate of biotinylated HepG2 cells as a source of receptor molecules. Our results showed that an approximately 1 lOkDa protein （p110） was detected to bind to preS1. Tissue distribution analysis further indicated that pll0 binding is restricted to human hepatocytes. The data demonstrated that p110 is a binding protein to preS1 on HepG2 cell membrane.

关 键 词：乙型肝炎病毒 前S1蛋白 结合蛋白 PULL down 

分 类 号：R373[医药卫生—病原生物学]