内源信号光学成像观测依达拉奉对脑损伤后SD大鼠损伤区微循环改变的影响  

Influence of edaravone on microcirculation in injuried area of cerebral injury SD rats by optical intrinsic signal imaging （OISI） observation

作　　者：周倩[1] 王玉成[3] 兰纯娜[2] 张明铭[1] 蒋宇钢[1] 胡德文[3] 

机构地区：[1]中南大学湘雅二医院神经外科,长沙410011 [2]中南大学湘雅二医院康复科,长沙410011 [3]国防科技大学机电工程与自动化学院自动控制系

出　　处：《中华生物医学工程杂志》2009年第3期165-169,共5页Chinese Journal of Biomedical Engineering

基　　金：湖南省自然科学基金（07JJ5008）

摘　　要：目的采用内源信号光学成像（OISI）在体监测自由基清除剂依达拉奉对颅脑外伤后SD大鼠损伤区微循环的改变。方法健康雄性SD大鼠60只，完全随机法分为正常组、对照组（外伤组）和实验组（自由基清除剂依达拉奉治疗组），每组各20只。应用钢针刺人脑体感区制造大鼠脑损伤模型。实验组大鼠脑损伤后腹腔注射自由基清除剂依达拉奉（3mg／kg）；对照组只造模不使用药物；正常组大鼠不予以处理。观察（546±10）nm（绿光）及（605±10）nm（橙光）波长光束照射下皮层反射内源光信号变化的特征。结果对照组和实验组40只大鼠全部造模成功。在OISI实验中，正常组20只均采集到有效数据，而对照组12只（绿光和橙光光束照射下各为6只）及实验组8只（绿光3只，橙光5只）采集到有效数据。正常组大鼠的反射光强灰度的变化曲线较平缓。对照组出现了大量的波峰。实验组曲线分为4个阶段：P1阶段出现1个波峰，增量约为基线水平的（5．7±3．5）％，时间宽度约为2．5min；P2阶段反射光强随时间的延长逐步升高，持续约1h；P3阶段反射光强随时间逐渐下降，持续约为40min；P4阶段出现的5个波峰的增量分别为基线水平的（7．1±3．2）％、（8．3±1．8）％、（10．7±1．5）％、（9．9±1．6）％和（7．4±2．0）％，时间宽度分别为1．5、2．5、4．4、4．2min和4min。3组反射光强灰度的差异具有统计学意义（F=6．050，P〈0．05）。刺激正常大鼠的后肢，其体感区功能响应持续时间为7-8s；而损伤后大鼠体感区刺激响应持续时间延长为11-15s；实验组使用依达拉奉后，体感区功能响应同正常大鼠的过程基本相同，但持续时间有所减少，为5～5．5s。结论自由基清除剂依达拉奉对损伤区脑组织的供血调控起较强的保护效果，可显著改善损伤后脑体感区的血液循环，可在Objective To monitor the changes of microcirculation after free radical scavenger edaravone treatment by optical intrinsic signal imaging （OISI） in injuried area of cerebral injury SD rats in vivo. Methods Sixty male SD rats were randomly divided into 3 groups： normal group （n=20） , control group （injury group, n=20） and experimental group （free radical scavenger edaravone-treating group, n= 20）. A steel needle was penetrated into cerebral somatosensory area in rats to establish cerebral injury animal models. The rats in experimental group were intraperitoneal injected with free radical scavenger edaravone （3 mg/kg） after cerebral injruy. Only operation without administration was performed in control group and the rats in normal group did not receive any treatment. Change character of optical intrinsic signals were recorded in the exposed cortex of all the rats with light illumination using （546± 10） nm and （605± 10） nm wavelength. Results All the rat models in control group and experimental group were successfully established. Effective dada were acquired from 20 rats in normal group, 12 rats in control group [6 rats in（546±10） nm wavelength and 6 rats in （605± 10） nm wavelength] , and 8 rats in experimental group [3 rats in （546± 10） nm wavelength and 5 rats in （605± 10） nm wavelength] in OISI experiment. The gray scale change curve of reflected light intensity in normal group was fairly gentle. The curve of control group had lots of wave crests. The curve of experimental group had 4 stages. There was a wave crest in PI stage and its amplitude was （5.7± 3.5）% of baseline. P1 stage lasted for 2.5 minutes. Reflected light intensity increased with time in P2 stage and lasted for 1 hour. Reflected light intensity decreased with time in P3 stage and lasted for 40 minutes. The amplitudes of five wave crests in P4 stage were （7.1±3.2）%, （8.3±1.8）%, （10.7±1.5）%, （9.9±1.6）% and （7.4±2.0）% of baseline respectively. The durations of wave cre

关 键 词：脑损伤 自由基清除剂 微循环 光学成像 依达拉奉 

分 类 号：R651.1[医药卫生—外科学]