不同剂量的氯胺酮对大鼠免疫功能和肿瘤转移的影响  

Effects of Different Doses of Ketamine on Immune Function and Tumor Metastasis in Rats

作　　者：沈智文[1] 李艳萍[1] 李杰[1] 王飞[1] 郑伟[2] 王旭东[2] 

机构地区：[1]中山大学附属第二医院麻醉科,广东广州510120 [2]肿瘤防治中心麻醉科,广东广州510060

出　　处：《中山大学学报（医学科学版）》2009年第A04期19-22,27,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2005B30301001);广东省医学科研项目(A2005249)

摘　　要：【目的】探讨不同剂量的氯胺酮对大鼠免疫功能和肿瘤转移的影响。【方法】Fischer344雄性大鼠100只随机分为5组:生理盐水组(Saline组);氯胺酮25mg/kg组(K25组);氯胺酮50mg/kg组(K50组);氯胺酮100mg/kg组(K100组);氯胺酮200mg/kg组(K200组),每组20只。予腹腔注射给药处理,24h后各组随机抽取10只大鼠进行检测淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+、NK细胞(CD161a+)的数量及NK细胞活性,而余下大鼠予尾静脉注入MADB106肿瘤细胞,于3周后测大鼠的肺转移瘤的数量。【结果】与Saline组相比,K25组CD3+、CD4+、CD8+、CD4+/CD8+没有明显变化,而K200组均明显降低(P<0.01),K50、K100组淋巴细胞亚群CD3+、CD4+亦明显亦降低(P<0.05或P<0.01);与Saline组相比,所有氯胺酮组大鼠CD161a+的数量和NK活性明显下降(P<0.01),而肺肿瘤转移明显增多(P<0.01)。氯胺酮剂量与大鼠NK细胞活性的Pearson相关系数为-0.801,氯胺酮剂量与大鼠NK细胞数量的Pearson相关系数为-0.420,氯胺酮剂量与大鼠肺肿瘤转移瘤的Pearson相关系数为0.969。【结论】氯胺酮能够抑制机体的免疫功能,从而促进肿瘤转移的易感性,NK细胞活性及肺肿瘤转移发生率与氯胺酮剂量相关。[Objective] To investigate the effects of different doses of ketamine on the immune function and tumor metastasis in rats. [Methods] 100 Fischer344 male rats were randomly assigned into 5 groups： Normal saline group（group Saline）, ketamine 25 mg/kg group（group K25）, ketamine 50 mg/kg group（group K50）, ketamine100 mg/kg group（group K100）, ketamine 200 mg/kg group（group K200）. 24 hours after intraperitoneal injection of ketamine or normal saline, 10 rats in each group were picked up randomly to detect of lymphocyte subsets CD3^＋,CD4^＋,CD8^＋,CD4^＋/CD8^＋ and NK cell counts, NK cell activity, The other 10 rats in each group were intravenous injected MADB106 tumor cells. Tumor lung metastases were assessed three weeks later. [Results] Compare to group Saline, the numbers of CD3^＋,CD4^＋,CD8^＋ lymphocytes and the ratios of CD4^＋/CD8^＋ showed no significant different in group K25, but decreased significantly in group K200 （P 〈 0.01）,and the numbers of CD3^＋,CD4^＋ lymphocytes were reduced significantly in group K50 and K100 （P 〈 0.05 or P 〈 0.01）. Compare to group Saline, the numbers of CD161a^＋ and NK cell activity of rats decreased significantly in all groups dealt with ketamine（P 〈 0.01 ）, while tumor metastases in lung were were dramatically increased （P 〈 0.01）. The Pearson correlation coefficient between the doses of ketamine and NK cell activity, NK cell counts, tumor Lung metastases is -0.801 .-0.420 and 0.969 separately. [Conclusion] Ketamine can suppress the immune function of rat, thus contribution to the susceptibility of tumor metastasis. NK cell activity and tumor Lung metastases are closely related with ketamine doses.

关 键 词：氯胺酮 免疫监视 肿瘤转移 

分 类 号：R971.2[医药卫生—药品]