HPLC法测定痔炎消颗粒中芦丁的含量  

Determination the Content of Rutin in the Zhiyanxiao Keli by HPLC

作　　者：方铁铮 符素平 

机构地区：[1]广州星群(药业)股份有限公司,广东广州510288

出　　处：《中国实验方剂学杂志》2009年第9期14-15,共2页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：建立测定痔炎消颗粒中芦丁含量的方法。方法：采用高效液相色谱法。色谱柱为Merck Lichrospher C18柱（250 mm×4.6 mm,5μm）;流动相为甲醇-1%冰乙酸（32∶68）;流速1 mL.min-1;检测波长为257 nm。结果：芦丁回归方程为Y=1 865.5X＋101.61,r=0.999 5;线性范围为0.299~8.970μg（n=6,RSD=0.91%）,平均回收率为99.54%,RSD为0.91%。结论：所建方法简便可行,快速,可用于痔炎消颗粒的质量控制。Objective：An HPLC method was established for the determination of Rutin. Methods：Using Merck Lichrospher C18（5 μm, 250 mm × 4.6 mm） with the mobile phase consisiting of methanol-1% glacial acetic acid（32： 68） ; The detective wavelength was 257 nm; The flow rate was 1 mL·min^-1. Results： The standard curve was liner with the range of 0.27 -7.11 μg（n =6,RSD=0.91%）, r =0.9995. The average recovery was 99.54%,RSD 0.91%. Conclusion：The method is simple and rapid which can be used for the quality control of Zhiyanxiao Keli.

关 键 词：高效液相色谱法 痔炎消颗粒 芦丁 

分 类 号：R284.1[医药卫生—中药学]