早期使用三七总皂苷对大鼠脑出血炎症的影响  

Effect of Panax Notoginoside on Inflammatory Reation of Rats with Acute Intracerebral Hemorrhage

作　　者：徐枝芳[1] 唐璐[1] 陈志刚[1] 朱陵群[2] 扎娃[1] 高芳[1] 钟伟森[1] 陈路[1] 

机构地区：[1]北京中医药大学东方医院神经内科,北京100078 [2]北京中医药大学东直门医院中医脑病研究室,北京100700

出　　处：《中国实验方剂学杂志》2009年第9期77-80,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:早期使用三七总皂苷对大鼠脑出血(ICH)神经功能缺损和炎症介质IL-6(白细胞介素-6)、MMP-9(基质金属蛋白酶-9)的影响。方法:将SD大鼠随机分为假手术组、模型组和三七总皂苷组,采用胶原酶注射尾状核法制作脑出血模型,观察Zea Longa评分和脑出血死亡率;免疫组织化学染色法观察血肿周围IL-6和MMP-9的表达。结果:三七总皂苷组造模5 d时Zea Longa评分优于模型组(P<0.05),造模24 h和5 d的减分值亦优于模型组(P<0.01),且未增加脑出血死亡率;模型组48h和7 d时脑组织IL-6和MMP-9明显升高,三七总皂苷用药7 d可下调IL-6(P<0.05)和MMP-9(P<0.05)。结论:三七总皂苷可减轻大鼠脑出血后神经功能缺损,抑制IL-6和MMP-9的释放以改善神经元损伤可能是其作用机制之一。Objective： to discuss the effect of Panax notoginoside （PNS） on the neurologic impairment and inflammatory factors IL-6, MMP-9 of rats with acute intracerebral hemorrhage. Method：The SD rats were divided into 3 groups randomly： sham group, model group and Panax notoginoside group. An animal model of ICH was edtablished by injecting collagenase Ⅶ into the caudate nucleus, and then Zea longa score, ICH mortality and the contents of IL-6 and MMP-9 in rat brain were measured. Results：Longa score of PNS group are lower than that of model group at the 5^th day after ICH model establishment （P 〈 0.05）. The improvement of neurologic impairment of PNS group at the 5^th day compared with the 1^st day are greater than that of model group（ P 〈 0.01 ）. The ICH mortality rate between PNS group and the model group had no significant difference. The contents of IL-6 and MMP-9 in brain tissues around hematoma of modal group were significantly elevated at the 48^th hour（ P 〈 0.01 and P 〈 0.05）, and continued to rise till the 7^th day（ P 〈 0.05 and P 〈 0.01 ）. The inhibitive effect of PNS on the expressions of IL-6（ P 〈 0.05）, MMP-9 （ P 〈 0.05） in brain on the 7^th day was obvious. Conclusion： Panax notoginoside can promote recovery of neurologic impairment after ICH. One of the mechanisms may be that PNS could effectively inhibit IL-6 and MMP-9.

关 键 词：脑出血 白细胞介素-6 基质金属蛋白酶-9 三七总皂苷 神经功能缺损 

分 类 号：R285.5[医药卫生—中药学]