不同阶段糖尿病大鼠胃肠激素变化及凉润通络方的干预作用  

Changes of Gastrointestinal Hormone in Different Stages of Diabetes of Rats and Intervention of Liangrun Tongluo Recipe

作　　者：李佃贵[1] 戎士玲[1] 王凤丽[1] 张永健[1] 

机构地区：[1]河北医科大学中医院内分泌科

出　　处：《中医杂志》2009年第9期835-838,共4页Journal of Traditional Chinese Medicine

基　　金：国家中医药管理局中医药科学技术研究专项(04-05JP09)

摘　　要：目的探讨凉润通络方对糖尿病大鼠胃肠运动功能改善的机制。方法雄性Wistar大鼠随机共分为凉润通络组、西沙必利组、模型组、正常组,每组又分3个治疗阶段,即预防阶段、早期治疗阶段、治疗阶段。采用链脲佐菌素(STZ)注射制作糖尿病大鼠模型,造模成功后,不予降糖药控制血糖,分别饲养6周、12周和18周后,各组再给予灌胃治疗。灌胃6周后检测血浆中的胃动素(MOT)、胆囊收缩素(CCK)、生长抑素(SS)、血糖、体重。结果糖尿病模型组大鼠不同阶段血浆中的MOT和CCK含量随着时间的延长均逐渐升高,SS血浆含量和体重则逐渐降低。与模型组比较,凉润通络方组在3个阶段对MOT、CCK、SS及体重均有明显改善(P<0.05),而西沙必利组对SS及后两个阶段的体重无明显改善(P>0.05)。两组各阶段对血糖均无明显影响(P>0.05)。结论凉润通络方在改善糖尿病大鼠胃肠功能障碍方面具有优势。Objective To study the mechanism of Liangrun Tongluo Recipe （Recipe of cool and moistening herbs for removing obstruction of meridians and collaterals） in improving gastrointestinal function of diabetic rats. Methods Male Wistar rats were randomly divided into four groups.. Liangrun Tongluo Recipe group, cisapride group, model group and normal group. Every group had three treatment stages： prevention stage, early treatment stage and treatment stage. The diabetic rat models were made by streptozotocin （STZ） injection. The models were fed for 6 weeks, 12 weeks and 18 weeks respectively, and then they were given garage treatment. After garage for 6 weeks, plasma motilin （MOT）, cholecystokinin （CCK）, somatostatin （SS）, blood glucose and body weight were tested. Results The content of plasma MOT and CCK of the model group increased gradually, while body weight and the contents of SS decreased gradually. In Liangrun Tongluo Recipe group, MOT, CCK, SS and body weight in the three stage were all improved significantly （P〈0. 05） as compared with those in the model group, while in cisapride group, SS and body weight in 12 and 18 weeks were not improved significantly（P〉0.05）. Blood glucose of both groups in 6 weeks, 12 weeks, and 18 weeks was not affected significantly,（P〉0. 05）. Conclusion Liangrun Tongluo Recipe has the superiority in improving the disturbance of the gastrointestinal function of diabetic rats.

关 键 词：凉润通络方 糖尿病胃肠功能障碍 胃动素 胆囊收缩素 生长抑素 

分 类 号：R285.5[医药卫生—中药学]