离子色谱抑制电导测定尿液中草酸根、磷酸根及枸橼酸根离子  

Determination of oxalate,phosphate and citrate in human urine by ion chromatography with suppressor conductivity detection

作　　者：陈梅兰[1] 叶明立 范云场[3] 朱岩[3] 

机构地区：[1]浙江树人大学生物与环境工程学院,杭州310015 [2]戴安中国有限公司杭州实验室,杭州310028 [3]浙江大学化学系,杭州310028

出　　处：《药物分析杂志》2009年第9期1466-1469,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(No.20775070);浙江省自然科学基金(No.Y507252);浙江省分析测试基金(No.2007F70061)

摘　　要：目的:建立一种离子色谱梯度淋洗抑制电导法快速测定尿液中草酸根、磷酸根及枸橼酸根离子浓度。方法:采集新鲜的尿样,经高速离心过0.2μm的微孔滤膜、On-Guard H小柱及C18预处理小柱后直接进样。选用Dionex IonPac AG23(50mm×4mm)阴离子保护柱和Dionex IonPac AS23(250mm×4mm)阴离子分析柱对样品进行分离,氢氧化钾溶液梯度淋洗,自再生抑制电导检测器检测。结果:线性范围为0.5～50μg·mL-1,r≥0.9998;日内精密度RSD≤3.6%,日间精密度RSD≤6.9%;样品加标平均回收率为96.0%～100.2%。结论:该方法操作简便、灵敏,选择性高,适用于常规样品分析。Objective：To develop a simple method for the determination of oxalate,phosphate, citrate in human u- fine by ion chromatography with gradient elution and conductivity detection. Methods：Fresh collective urine sam- ples were centrifuged for 15 min at 5000 r · min -1 and then the above solution was filtered through a 0. 2 μm syr- inge membrane filter, On- Guard H column and C18 cleanup column before injection. The chromatographic separa- tion was performed on Dionex IonPac AG23 （50 mm× 4 mm） guard column, and an Dionex IonPac AS23 （250 mm × 4 mm） analytical column using potassium hydroxide as eluent with a serf - regeneration suppressor. Results： This novel method exhibited excellent regression（ r 〉10. 9998 ）in the range of 0. 5 -50μg·mL-1, satisfactory precision （RSD less than 3.6% within one day and less than 6. 9% day to day）. The recovery obtained in the accuracy test was in the range of 96.0% - 100. 2%. Conclusion： The method is simple, accurate, stable and precise. It can be easily and conveniently adopted for the routine quality control analysis.

关 键 词：离子色谱 尿液 草酸根 磷酸根 枸橼酸根 

分 类 号：R917[医药卫生—药物分析学]